Ultrafiltration Concentration
Ultrafiltration Concentration - Related Videos
02:49
Bacterial Culture Filtrate Protein Concentration using an Ultrafiltration Device
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2025
This video demonstrates the isolation and concentration of bacterial culture filtrate proteins, which include bacterial extracellular vesicles, using ultrafiltration. The concentrated extracellular vesicles serve as diagnostic markers for disease detection.
12:47
Tangential Flow Ultrafiltration: A “Green” Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles
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Cited by 20 •
2012
Tangential flow ultrafiltration (TFU) is a recirculation method used for the weight-based separation of biosamples. TFU was adapted to size-select (1-20 nm diameter) and highly concentrate a large volume of polydisperse silver nanoparticles (4 L of 15.2 μg ml-1 down to 4 ml of 8,539.9 μg ml-1) with minimal aggregation.
10:53
Concentration of Virus Particles from Environmental Water and Wastewater Samples Using Skimmed Milk Flocculation and Ultrafiltration
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Cited by 2 •
2023
Virus concentration from environmental water and wastewater samples is a challenging task, carried out primarily for the identification and quantification of viruses. While several virus concentration methods have been developed and tested, we demonstrate here the effectiveness of ultrafiltration and skimmed milk flocculation for RNA viruses with different sample types.
10:19
Three-Dimensionally Printed Microfluidic Cross-flow System for Ultrafiltration/Nanofiltration Membrane Performance Testing
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Cited by 9 •
2016
Design and fabrication of a three-dimensionally (3-D) printed microfluidic cross-flow filtration system is demonstrated. The system is used to test performance and observe fouling of ultrafiltration and nanofiltration (thin film composite) membranes.
02:41
Concentration Cells
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2020
A concentration cell is a type of a voltaic cell constructed by connecting two almost identical half-cells, both based on the same half-reaction and using the same electrode, differing only in the concentration of one redox species. A concentration cell's potential, therefore, is determined only by the concentration difference of the particular redox species. Consider the following voltaic cell: From the given information, the cell potential of this concentration cell can be calculated using...
FAQs
The membrane’s molecular-weight cutoff determines which molecules remain in the retentate and which pass through with water and small solutes. A cutoff selected relative to the target material therefore controls recovery of proteins, nucleic acids, viruses, or other macromolecules. This choice directly affects how effectively the reduced-volume sample supports later detection or analysis.
Pressure or centrifugal force supplies the driving force that moves water and small solutes across a semipermeable membrane. Larger molecules are retained, so the applied force and membrane selectivity together determine how rapidly sample volume decreases without transferring the target macromolecular material into the passing liquid.
Small solutes leave with the water that crosses the membrane, whereas larger target molecules remain in the retentate. This selective movement allows the method to do more than reduce volume: it can exchange the sample environment, including buffers, while preserving concentrated macromolecular material for subsequent biochemical work.
A typical workflow places the dilute biological sample against a semipermeable membrane, applies pressure or centrifugal force, and allows water and small solutes to pass through. The retained fraction is then recovered as the concentrated sample. That material can be directed to detection, electrophoresis, chromatography, or biochemical assays, depending on the experimental goal.
Suitability depends primarily on the semipermeable membrane’s molecular-weight cutoff and the size of the biological material that must be retained. The applied pressure or centrifugal force provides movement through the membrane, while the cutoff determines whether desired macromolecules remain available for downstream analysis rather than leaving with the passing liquid.
It is especially useful when a biological sample is dilute but contains valuable macromolecules that must be handled efficiently. Concentrating proteins, nucleic acids, viruses, or related materials can improve detection and prepare limited samples for electrophoresis, chromatography, or biochemical assays. The approach also fits purification workflows where buffer exchange or reduced sample volume is needed.