A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 02:23Synthesis of Colloidal AgNPs
- 04:07UV-Vis Spectrophotometry: Surface Plasmon Resonance
- 05:17Tangential Flow Ultrafiltration: Size-selection and Concentration
- 07:17Inductively Coupled Plasma Optical Emission Spectroscopy: Quantification of Silver
- 08:37Size Distribution of Colloidal AgNPs via Transmission Electron Microscopy (TEM)
- 09:22Results: Synthesis and Characterization of Colloidal AgNPs
- 11:43Conclusion
Tangential flow ultrafiltration (TFU) is a recirculation method used for the weight-based separation of biosamples. TFU was adapted to size-select (1-20 nm diameter) and highly concentrate a large volume of polydisperse silver nanoparticles (4 L of 15.2 μg ml-1 down to 4 ml of 8,539.9 μg ml-1) with minimal aggregation.
Tags
Tangential Flow UltrafiltrationColloidal Silver NanoparticlesAgNPsSize SelectionConcentrationNanoparticle ApplicationsAntimicrobial PropertiesControlled FabricationSize-based IsolationChemically Aggressive Capping/stabilizing AgentsOrganic SolventsToxicity Of ReagentsHigh CostsReduced EfficiencyAgNP SynthesisIsolation MethodsCentrifugationSize-dependent SolubilitySize-exclusion ChromatographyTFU (Tangential Flow Ultrafiltration)Creighton AgNPsUltracentrifugationRecirculation MethodHollow Fiber MembranesPore Size
