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Concentration of Virus Particles from Environmental Water and Wastewater Samples Using Skimmed Milk Flocculation and Ultrafiltration
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JoVE Journal Environment
Concentration of Virus Particles from Environmental Water and Wastewater Samples Using Skimmed Milk Flocculation and Ultrafiltration

Concentration of Virus Particles from Environmental Water and Wastewater Samples Using Skimmed Milk Flocculation and Ultrafiltration

DOI:
10.3791/65058-v

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10:53 min

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March 17, 2023

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, , , ,
1Department of Civil Engineering, Price Faculty of Engineering,University of Manitoba, 2Department of Microbiology, Faculty of Science,University of Manitoba

Chapters

  • 00:04Introduction
  • 01:23Comparison of Serial Ultrafiltration and Skimmed Milk Flocculation to Concentrate Viruses in Wastewater Samples
  • 04:53Filtration and Concentration of DNA and RNA Viruses for Water‐Based Epidemiology
  • 07:28Direct Precipitation and Concentration of Microbial Fractions for Water‐Based Epidemiology
  • 08:54Results: Effectiveness of Ultrafiltration and Skimmed Milk Flocculation for RNA Viruses with Different Sample Types
  • 10:10Conclusion

Summary

Automatic Translation

Automatic Translation

Virus concentration from environmental water and wastewater samples is a challenging task, carried out primarily for the identification and quantification of viruses. While several virus concentration methods have been developed and tested, we demonstrate here the effectiveness of ultrafiltration and skimmed milk flocculation for RNA viruses with different sample types.

Tags

Keywords: Virus ConcentrationWastewater EpidemiologySkimmed Milk FlocculationUltrafiltrationMetagenomicsPCRWastewater SamplesArmored RNAPH AdjustmentCentrifugation

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