Upright Microscope
Upright Microscope - Related Videos
07:34
Upright Imaging of Drosophila Embryos
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Cited by 7 •
2010
Here we present a mounting protocol for stained Drosophila embryos in an upright position that allows imaging of cross-sections using Confocal microscopy.
12:29
Upright Imaging of Drosophila Egg Chambers
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Cited by 1 •
2015
The upright imaging method described in this protocol allows for the detailed visualization of the poles of a developing Drosophila melanogaster egg. This end-on view provides a new perspective into the arrangements and morphologies of multiple cell types in the follicular epithelium.
09:47
Using Ex Vivo Upright Droplet Cultures of Whole Fetal Organs to Study Developmental Processes during Mouse Organogenesis
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Cited by 13 •
2015
The ex vivo upright droplet culture is an alternative to current in vitro and in vivo experimental techniques. This protocol is easy to perform and requires smaller amounts of reagent, while permitting the ability to manipulate and study fetal vascularization, morphogenesis, and organogenesis.
08:13
Preparation of Murine Submandibular Salivary Gland for Upright Intravital Microscopy
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Cited by 9 •
2018
We describe a protocol to surgically expose and stabilize the murine submandibular salivary gland for intravital imaging using upright intravital microscopy. This protocol is easily adaptable to other exocrine glands of the head and neck region of mice and other small rodents.
04:57
Proper Care and Cleaning of the Microscope
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Cited by 2 •
2008
Keeping the microscope optics clean is important for high-quality imaging. Dust, fingerprints, excess immersion oil, or mounting medium on or in a microscope causes reduction in contrast and resolution. DIC is especially sensitive to contamination and scratches on the lens surfaces. This protocol details the procedure for keeping the microscope clean.
FAQs
The illumination path determines how light interacts with the specimen before the objective forms an image. Transmitted illumination sends light through a sample, while reflected illumination uses light interacting with the sample surface or exposed structures. This choice allows the same microscope arrangement to accommodate different biological preparations and supports observation of structures in slides or containers.
The objective is the primary imaging element: it forms a magnified image from light affected by the specimen. An eyepiece further enlarges that image for direct visual observation, whereas a camera records the image for imaging-based examination. Interchangeable objectives and imaging accessories therefore let researchers adapt observation and documentation to different biological samples.
Focusing changes the distance between the lens and specimen, bringing selected structures into sharp view. This adjustment is essential when examining fine cellular features, tissue organization, or microorganisms because an unfocused image can obscure boundaries and internal patterns. Careful focus consequently supports more reliable visual assessment of morphology and spatial arrangement.
An upright microscope is particularly compatible with specimens mounted on slides or held in containers, formats commonly used for prepared sections, cells, and microorganisms. The sample can be positioned beneath the objective while light is transmitted through or reflected from it. This arrangement supports examination of both prepared material and samples maintained in suitable containers.
A basic workflow begins by placing the specimen on a slide or in a suitable container, selecting an appropriate objective, and choosing transmitted or reflected illumination according to how light should interact with the sample. The user then adjusts the lens-to-specimen distance until structures appear sharp and observes them through the eyepiece or records them with a camera.
In biology, observations can reveal morphology, meaning the form and visible features of cells, tissues, and microorganisms, as well as their organization within prepared sections. The instrument can also support examination of responses to experimental conditions. These outcomes make it useful for comparing structural appearance and arrangement across biological samples or treatments.
Interchangeable objectives and imaging accessories are valuable when a study requires different viewing arrangements or documentation methods across samples. Objectives support examination through the microscope's optical system, while an eyepiece or camera provides visual viewing or image capture. Together, these components extend the instrument's usefulness across teaching, research, and varied biological preparations.