Oleic Acid Emulsion
Oleic Acid Emulsion - Related Videos
10:42
A Tripeptide-Stabilized Nanoemulsion of Oleic Acid
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2019
This protocol describes an efficient method to synthesize a nanoemulsion of an oleic acids-platinum(II) conjugate stabilized with a lysine-tyrosine-phenylalanine (KYF) tripeptide. The nanoemulsion forms under mild synthetic conditions via self-assembly of the KYF and the conjugate.
04:10
A Porcine Model of Acute Respiratory Failure with a Continuous Infusion of Oleic Acid
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Cited by 1 •
2024
Infusing oleic acid continuously into the pulmonary artery of an anesthetized adult pig induces acute respiratory failure, enabling controlled experimentation during acute respiratory decompensation.
04:45
Mouse Model of Oleic Acid-Induced Acute Respiratory Distress Syndrome
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Cited by 4 •
2022
The present protocol describes a lung injury model in mice using oleic acid to mimic acute respiratory distress syndrome (ARDS). This model increases the inflammatory mediators on edema and decreases lung compliance. Oleic acid is used in the salt form (oleate) since this physiological form avoids the risk of embolism.
06:06
Oleic Acid-Injection in Pigs As a Model for Acute Respiratory Distress Syndrome
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Cited by 20 •
2018
In this article, we present a protocol to induce acute lung injury in pigs by central-venous injection of oleic acid. This is an established animal model for studying the acute respiratory distress syndrome (ARDS).
08:35
Minimum Burning Pressures of Water-based Emulsion Explosives
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2017
We present an apparatus based on hot-wire ignition in a pressurized enclosure and an associated methodology to measure the minimum pressure required to induce sustained combustion in water-based emulsion explosives. This method improves product characterization to allow one to use them more safely during pumping and mixing operations.
FAQs
Emulsifiers stabilize the boundary between oleic acid and water, helping droplets remain distributed during an experiment. Mechanical mixing or homogenization creates the dispersion, while the emulsifier supports the resulting oil–water interface. This matters because uneven separation can change how much oleic acid contacts cells or assay components, making biological responses harder to compare across samples.
Droplet size influences oleic acid distribution and its contact with cells, tissues, or other assay components. Smaller or more consistently distributed droplets may expose biological material differently from larger, unstable droplets. Consequently, researchers should treat droplet size as an experimental variable, because differences in size can contribute to changes in fatty acid uptake, lipid metabolism, or cellular stress.
Emulsifier composition, oleic acid concentration, droplet size, and overall stability can each affect experimental outcomes. These variables determine how consistently the fatty acid remains distributed and how it contacts biological material. Controlling them is essential when comparing samples, since an apparent cellular response may reflect differences in emulsion properties rather than the intended lipid exposure alone.
Preparation requires combining oleic acid with an aqueous phase and an appropriate emulsifier, followed by mechanical mixing or homogenization to disperse the fatty acid. The resulting preparation should be characterized for relevant properties such as droplet size, emulsifier composition, concentration, and stability. This workflow supports more uniform exposure and improves reproducibility between biological experiments.
The preparation supports studies of fatty acid uptake, lipid metabolism, cellular stress, and responses to elevated lipid exposure. Its value comes from making oleic acid more consistently available to cells, tissues, or assay components. Researchers can therefore examine how biological systems respond when lipid availability changes, while relating those outcomes to the emulsion’s controlled properties.
Observed effects should be considered alongside the emulsion’s concentration, droplet size, emulsifier composition, and stability. These features can alter oleic acid distribution and contact with cells, so they may influence uptake, metabolism, or stress responses. Recording and controlling such parameters helps distinguish a biological response to elevated lipid exposure from variability introduced during emulsion preparation.