Ureter Isolation

Ureter isolation is a laboratory and surgical dissection technique used to separate the ureter, the muscular tube that carries urine from the kidney to the bladder, from surrounding tissues for examination or further manipulation. The procedure relies on identifying the ureter by its anatomical position and carefully dissecting along its outer surface while preserving the tubular wall, blood supply, and adjacent structures. Isolated ureters support studies of urinary tract anatomy, smooth-muscle contraction, epithelial function, drug responses, and disease-related changes. In research and teaching, the technique provides a defined preparation for microscopy, physiological assays, and experimental modeling of ureteral obstruction or transport.

Ureter Isolation - Related Videos

Education

JoVE Core - Anatomy and Physiology

Ureters

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2024

The ureters are retroperitoneal tubes located on either side of the vertebral column. They are responsible for transporting urine from each kidney to the urinary bladder. These tubes have thick walls and are approximately 25-30 cm long. Their diameter is around 10 mm at the renal pelvis, gradually narrowing to 1 mm as the ureter obliquely enters the posterior bladder wall through the ureteric orifices. The shape of these orifices is slit-like, which helps to prevent urine backflow toward the...

Anatomy of the Genitourinary System I: Kidneys and Ureters

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2025

The upper urinary system comprises two kidneys and two ureters, which are crucial in filtering blood and forming urine.KidneysLocation and Structure:The kidneys are two bean-shaped organs positioned behind the peritoneum on either side of the spine.Kidneys are between the 12th thoracic (T12) and the 3rd lumbar (L3) vertebrae.The position of the liver causes the right kidney to sit slightly lower than the left.Protective Layers:Each kidney is enveloped in a tough, fibrous membrane called the...

Imaging Studies I: Kidney, Ureter, and Bladder Studies

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2025

Kidney, Ureter, and Bladder (KUB) StudiesKidney, Ureter, and Bladder (KUB) studies are standard diagnostic imaging procedures used to assess the anatomy of the urinary system. They are commonly utilized for patients experiencing abdominal pain or urinary symptoms. By using a simple X-ray of the abdomen, KUB studies can reveal structural and pathological abnormalities within the kidneys, ureters, and bladder. These studies are particularly valuable in diagnosing kidney stones, urinary...

Research

JoVE Journal - Biology

The Mouse Isolated Perfused Kidney Technique

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Cited by 24 •

2016

The mouse isolated perfused kidney (MIPK) is a technique for keeping a mouse kidney under ex vivo conditions perfused and functional for 1 hr. The buffers and surgical technique are described in detail.

Malpighian Tubule Dissection from an Adult Fly: Isolating an Osmoregulatory and Excretory Organ

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2023

This article describes the dissection of the anterior Malpighian tubules (MT) from a female Drosophila fly and an example protocol from Rossano et al. (2017) in which the MTs are prepared for live imaging in a perfusion chamber.

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