Ve-cadherin Localization
Ve-cadherin Localization - Related Videos
01:25
Structure of Cadherins
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2023
The cadherins were one of the first cell adhesion molecules discovered; the term “cadherins” is based on their calcium-dependent adhering properties. The first cadherins discovered on the epithelial, neuronal, and placental cells were named E-cadherin, P-cadherin, and N-cadherin, respectively. These classical cadherins share sequence and structural similarities. Other cadherins, including those involved in cell signaling, are grouped into non-classical cadherins. This diversity of cadherins...
01:19
Cadherins in Tissue Organization
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2023
The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues. Cell Sorting During Development Cell sorting plays an...
11:31
Analysis of Protein-protein Interactions and Co-localization Between Components of Gap, Tight, and Adherens Junctions in Murine Mammary Glands
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Cited by 5 •
2017
Intercellular junctions are requisites for mammary gland stage-specific functions and development. This manuscript provides a detailed protocol for the study of protein-protein interactions (PPIs) and co-localization using murine mammary glands. These techniques allow for the investigation of the dynamics of the physical association between intercellular junctions at different developmental stages.
13:13
Nano-fEM: Protein Localization Using Photo-activated Localization Microscopy and Electron Microscopy
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Cited by 14 •
2012
We describe a method to localize fluorescently tagged proteins in electron micrographs. Fluorescence is first localized using photo-activated localization microscopy on ultrathin sections. These images are then aligned to electron micrographs of the same section.
07:06
Visualizing RNA Localization in Xenopus Oocytes
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Cited by 5 •
2010
Visualization of in vivo RNA transport is accomplished by microinjection of fluorescently labeled RNA transcripts into Xenopus oocytes, followed by confocal microscopy.
FAQs
Calcium-dependent homophilic binding allows Ve-cadherin molecules on neighboring endothelial cells to connect with one another. This interaction supports adhesion precisely where cells meet and contributes to the organization of adherens junctions. Examining whether Ve-cadherin remains concentrated at these contact sites helps researchers evaluate how effectively endothelial cell-cell contacts are maintained.
The cytoplasmic domain of Ve-cadherin associates with catenins and the actin cytoskeleton, linking the adhesion complex to the cell’s structural framework. This connection helps stabilize endothelial junctions rather than leaving adhesion dependent only on extracellular binding. Studying this association provides a mechanistic view of how junctional organization supports vascular barrier integrity.
A redistribution of Ve-cadherin can signal that endothelial junctions are being remodeled rather than remaining structurally constant. In biological studies, such changes may be examined in relation to altered vascular permeability, inflammation, or angiogenesis. Localization therefore serves as a spatial readout that connects junction organization with broader changes in endothelial function.
Ve-cadherin localization provides information about the organization of endothelial cell contacts that contribute to vessel integrity. A study can use its distribution to assess whether junctional structures remain organized or show patterns consistent with remodeling. This makes localization relevant to investigations of barrier regulation, including conditions associated with permeability changes and inflammatory vascular responses.
Researchers commonly assess Ve-cadherin distribution with fluorescence microscopy, immunostaining, and related imaging methods. These approaches allow the protein’s spatial pattern to be visualized in relation to endothelial cell contacts. The resulting images can then support evaluation of junctional organization and comparison of localization patterns across biological conditions being investigated.
This imaging-based analysis is useful for studying endothelial barrier function, vessel development, and disease mechanisms. Researchers can examine whether localization patterns change during junctional remodeling, altered permeability, inflammation, or angiogenesis. Because the readout is spatial, it connects molecular organization at endothelial contacts with biological processes affecting vascular structure and behavior.