Biology
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Nano-fEM: Protein Localization Using Photo-activated Localization Microscopy and Electron Microscopy
Chapters
Summary December 3rd, 2012
We describe a method to localize fluorescently tagged proteins in electron micrographs. Fluorescence is first localized using photo-activated localization microscopy on ultrathin sections. These images are then aligned to electron micrographs of the same section.
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