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JoVE Journal
Biology

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Nano-fEM: Protein Localization Using Photo-activated Localization Microscopy and Electron Microscopy

Article DOI: 10.3791/3995
December 3rd, 2012

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Summary December 3rd, 2012

We describe a method to localize fluorescently tagged proteins in electron micrographs. Fluorescence is first localized using photo-activated localization microscopy on ultrathin sections. These images are then aligned to electron micrographs of the same section.

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