Viral Rna Silencing Suppressor
Viral Rna Silencing Suppressor - Related Videos
10:19
Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens
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Cited by 2 •
2020
Here, we present a modified screening method that can be extensively used to quickly screen RNA silencing suppressors in plant pathogens.
02:12
Co-Infiltration Assay for Screening Pathogen Effectors that Suppress Plant RNA Silencing
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2026
Source: Shi, J., et al. Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens. J. Vis. Exp. (2020)This video demonstrates a co-infiltration assay for identifying pathogen effector proteins that suppress RNA silencing in Nicotiana benthamiana 16c. A fluorescent reporter and a candidate effector gene are delivered into plant leaves via Agrobacterium. Normally, RNA silencing degrades reporter transcripts, reducing fluorescence. Suppressor effectors...
02:15
Agrobacterium-Mediated Delivery of Recombinant Viral Clones into Maize Seedlings for Gene Silencing
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2026
Source: Beernink, B. M. et al. Direct Agroinoculation of Maize Seedlings by Injection with Recombinant Foxtail Mosaic Virus and Sugarcane Mosaic Virus Infectious Clones. J. Vis. Exp. (2021)This video demonstrates the Agrobacterium-mediated delivery of recombinant viral clones into maize seedlings for gene silencing. It outlines the process of Agrobacterium infection, systemic viral spread, and RNA interference-driven phenotypic changes.
08:36
Chitosan/Interfering RNA Nanoparticle Mediated Gene Silencing in Disease Vector Mosquito Larvae
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Cited by 94 •
2015
Here we describe a procedure for inhibiting gene function in disease vector mosquitoes through the use of chitosan/interfering RNA nanoparticles that are ingested by larvae.
10:16
Detection of Viral RNA by Fluorescence in situ Hybridization (FISH)
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Cited by 35 •
2012
A fluorescence in situ hybridization (FISH) method was developed to visually detect viral genomic RNA using fluorescence microscopy. A probe is made with specificity to the viral RNA that can then be identified using a combination of hybridization and immunofluorescence techniques. This technique offers the advantage of identifying the localization of the viral RNA or DNA at steady-state, providing information on the control of intracellular virus trafficking events.
FAQs
Binding double-stranded RNA can interrupt RNA silencing before viral RNA-derived signals are fully processed. By associating with this RNA form, a suppressor may prevent the pathway from generating or accessing the small interfering RNAs needed for downstream silencing. This mechanism helps explain how a virus can reduce recognition and degradation of its RNA by the host cell.
Some suppressors act after small interfering RNAs have formed by binding or sequestering them. This prevents the molecules from participating normally in silencing, including their incorporation into Argonaute-containing RNA-induced silencing complexes. Studying this interaction distinguishes inhibition of small interfering RNA availability from inhibition at an earlier processing stage, providing a more detailed view of pathway control.
The targeted stage reveals which part of RNA silencing is most vulnerable to viral interference. A protein that affects double-stranded RNA processing acts differently from one that sequesters small interfering RNAs or blocks their entry into Argonaute-containing complexes. Mapping these differences connects a viral evasion strategy with a specific molecular step in host defense.
Viral RNA silencing suppressors provide a way to examine the relationship between viral immune evasion and cellular gene regulation. In plant and animal systems, they show how RNA silencing can restrict viral RNA while also participating in broader regulation of gene expression. Their study therefore links infection biology with fundamental mechanisms of RNA interference.
Researchers can use these proteins as pathway-dissection tools by examining which silencing stage is disrupted when a suppressor is present. Effects on double-stranded RNA, small interfering RNAs, or Argonaute-containing complexes help distinguish processing, sequestration, and incorporation mechanisms. This approach clarifies the organization of RNA silencing without treating the pathway as a single undifferentiated response.
Their study supports functional genomics by helping investigators analyze RNA interference and gene regulation. In crop protection and virus-resistant biotechnology, understanding how suppressors counter host defenses can inform strategies aimed at strengthening resistance or managing viral effects. These applications arise from connecting the proteins' molecular targets with the broader outcomes of viral RNA persistence and silencing control.