Wastewater Dna Extraction
Wastewater Dna Extraction - Related Videos
06:54
Enhanced Extraction of Low-Molecular Weight DNA from Wastewater for Comprehensive Assessment of Antimicrobial Resistance
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2024
Here, we present a simple technique to assess environmental antimicrobial resistance (AMR) by enhancing the proportion of low-molecular-weight extracellular DNA. Prior treatment with 20%-30% PEG and 1.2 M NaCl allows detection of both genomic and horizontally transferred AMR genes. The protocol lends itself to a kit-free process with additional optimization.
09:59
Community DNA Extraction from Bacterial Colonies
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2023
Source: Laboratories of Dr. Ian Pepper and Dr. Charles Gerba - The University of Arizona Demonstrating Author: Luisa Ikner Traditional methods of analysis for microbial communities within soils have usually involved either cultural assays utilizing dilution and plating methodology on selective and differential media or direct count assays. Direct counts offer information about the total number of bacteria present, but give no information about the number or diversity of populations present...
05:40
Comparison of Scale in a Photosynthetic Reactor System for Algal Remediation of Wastewater
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Cited by 3 •
2017
An experimental methodology is presented to compare the performance of small (100 L) and large (1,000 L) scale reactors designed for algae remediation of landfill wastewater. System characteristics, including surface area to volume ratio, retention time, biomass density, and wastewater feed concentrations, can be adjusted based on application.
09:30
Extraction of High Molecular Weight DNA from Microbial Mats
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Cited by 4 •
2011
We provide an improved protocol for extracting high molecular weight DNA from hypersaline microbial mats. Microbial cells are separated from the mat matrix prior to DNA extraction and purification. This enhances the concentrations, quality, and size of the DNA. The protocol may be used for other refractory samples.
06:58
Collection and Extraction of Saliva DNA for Next Generation Sequencing
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Cited by 45 •
2014
DNA extraction from saliva can provide a readily available source of high molecular weight DNA, with little to no degradation/fragmentation. This protocol provides optimized parameters for saliva collection/storage and DNA extraction to be of sufficient quality and quantity for downstream DNA assays with high quality requirements.
FAQs
Sample concentration is essential because wastewater often contains dilute genetic material spread through a large volume. Filtration or centrifugation gathers that material before purification, increasing the amount available for downstream analysis. This step helps convert a low-signal environmental sample into DNA that can be tested for biological targets.
These sample components make recovery and analysis more difficult. Solids and organic matter complicate isolation, while other substances may inhibit downstream testing if they remain with the extracted material. The workflow therefore treats concentration and purification as complementary steps: one gathers material, and the other removes interfering substances so PCR or sequencing can produce interpretable evidence.
Cell lysis breaks open cells so their genetic material becomes accessible for recovery and purification. This step connects concentrated wastewater material to the isolated DNA ultimately examined by PCR or sequencing. By releasing genetic material from cells, lysis supports detection of biological targets that would otherwise be less accessible during downstream measurement.
The workflow generally begins by concentrating material from wastewater through filtration or centrifugation. Cells are then lysed, and the resulting material undergoes purification to isolate DNA from solids, organic matter, and potential inhibitors. The purified extract proceeds to PCR or sequencing, creating a practical chain from complex sample collection to biological detection and community assessment.
After purification, PCR or sequencing provides the analytical stage of the workflow. These methods can identify pathogens, monitor antimicrobial-resistance genes, or assess microbial communities in the extracted material. Extraction is therefore not the final measurement; it prepares a complex environmental sample so genetic signals can be examined and related to biological activity or public-health trends.
In wastewater-based epidemiology, extracted DNA turns biological material in sewage into measurable evidence. Testing can support monitoring of pathogens, antimicrobial-resistance genes, and microbial communities, while the resulting measurements can inform understanding of public-health trends. The approach depends on obtaining purified DNA that remains suitable for analysis despite the complexity of the original sample.