High Throughput Workflow

A high throughput workflow is a coordinated process for analyzing many biological samples, reactions, or measurements efficiently and reproducibly, enabling researchers to generate large datasets in less time than conventional approaches. It typically combines standardized sample preparation, parallel processing in microplates or other formats, automated liquid handling, instrument-based detection, and software-assisted data analysis to reduce manual intervention and experimental variation. In biology, high throughput workflows support drug screening, genomics, proteomics, cell-based assays, and biomarker discovery, helping investigators compare conditions systematically, identify meaningful patterns, and prioritize experiments for further validation.

High Throughput Workflow - Related Videos

Research

JoVE Journal - Biology

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes

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2015

Here we describe a cell-based reporter gene assay as a valuable tool to screen chemical libraries for compounds modulating post-transcriptional control mechanisms exerted through 3’ UTR.

High-throughput Imaging and Analysis Workflow for Evaluating Skin Cell Phenotypes and Proliferation States in Tissue Samples

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Cited by 1 •

2025

The combination of iterative-bleaching-extends-multiplexity (IBEX) and a commercial nucleotide labeling assay (Click-iT EdU) enables the detection and categorization of dividing cell types in highly dynamic processes in fixed frozen murine tissue sections. Furthermore, a novel open-source image processing pipeline provides high-throughput image acquisition and analysis.

Research

JoVE Journal - Biochemistry
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High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

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Cited by 16 •

2023

This protocol details high-throughput crystallization screening, ranging from the 1,536 microassay plate preparation to the end of a 6 week experimental time window. Details are included about the sample setup, the imaging obtained, and how users can perform analyses using an artificial intelligence-enabled graphical user interface to quickly and efficiently identify macromolecular crystallization conditions.

Research

JoVE Journal - Biology
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A Quantitative Fitness Analysis Workflow

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Cited by 25 •

2012

Quantitative Fitness Analysis (QFA) is a complementary series of experimental and computational methods for estimating microbial culture fitnesses. QFA estimates the effect of genetic mutations, drugs or other applied treatments on microbe growth. Experiments scaling from focussed analysis of single cultures to thousands of parallel cultures can be designed.

Integrating Automated Simulation Workflows with 3D Visualization for Virtual Experiments in the Metaverse

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2026

A generalized, FAIR-compliant method is presented for domain-expert researchers seeking to integrate simulation and data-processing tools into automated workflows for 3D virtual experiments. A neutronics example demonstrates setting up a local Galaxy instance, wrapping OpenMC and file-conversion tools, launching workflows from Omniverse, and visualizing the converted 3D outputs.

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