Ishikawa Cells
Ishikawa Cells - Related Videos
13:06
Improved In-gel Reductive β-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry
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Cited by 6 •
2014
In order to comprehensively explore the diversity of O-linked glycans, a new procedure for in-gel reductive β-elimination, combined with permethylation and a rapid phase-partition method, is applied to the analysis of O-linked glycans directly released from glycoproteins resolved by SDS-PAGE and amenable to subsequent glycomic analysis by mass spectrometry.
09:58
A Choroid Plexus Epithelial Cell-based Model of the Human Blood-Cerebrospinal Fluid Barrier to Study Bacterial Infection from the Basolateral Side
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Cited by 45 •
2016
The epithelial cells of the choroid plexus (CP) form the blood-cerebrospinal fluid barrier (BCSFB). An in vitro model of the BCSFB employs human choroid plexus papilloma (HIBCPP) cells. This article describes culturing and basolateral infection of HIBCPP cells using a cell culture filter insert system.
10:04
Tractable In Vivo Reprogramming of Tumor Cells to Type 1 Conventional Dendritic Cell-like Cells
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2025
This protocol describes the in vivo reprogramming of mouse cancer cells into type 1 dendritic-like cells within the tumor microenvironment through enforced expression of the transcription factors PU.1, IRF8, and BATF3.
22:06
Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
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Cited by 8 •
2007
This protocol details the derivation of transplantable hematopoietic stem cells from mouse embryonic stem cells (ESC) and their subsequent injection into lethally irradiated recipient mice. Briefly, ESC are differentiated as embryoid bodies, which are then infected with retroviral HoxB4 and co-cultured with OP9 stromal cells and hematopoietic cytokines.
02:47
Human ES cells: Starting Culture from Frozen Cells
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2006
Here we demonstrate how our lab begins a HuES human embryonic stem cell line culture from a frozen stock.
FAQs
Steroid hormones such as estrogen and progesterone act through their receptors to produce changes in gene expression. Those molecular changes can influence cellular differentiation and the secretion of endometrial factors, connecting hormone exposure with epithelial behavior. This receptor-mediated response makes the cells useful for examining how hormonal signals may regulate uterine tissue functions under controlled culture conditions.
Changes in gene expression provide a molecular indication of hormone response, while altered differentiation reflects a shift in epithelial state. Secretion of endometrial factors supplies an additional functional outcome. Considering these measures together can help connect receptor-mediated signaling with tissue-related behavior rather than relying on a single cellular observation.
Their reproducible growth and epithelial characteristics support controlled experiments, but they do not by themselves establish how normal human endometrium behaves. Findings should therefore be compared with primary endometrial cells and in vivo models. This comparison helps identify which hormone responses may reflect general biology and which may depend on the cancer-derived cell line.
In studies of endometrial receptivity and implantation-related signaling, researchers can expose cultured cells to relevant hormonal conditions and examine resulting molecular, differentiation, or secretory changes. The model also supports investigations of reproductive disease. These applications allow specific signaling responses to be studied in a controlled epithelial system before conclusions are extended to more complex biological settings.
Controlled culture studies with Ishikawa Cells can examine responses to steroid hormones or potential therapeutics, followed by assessment of gene expression, cellular differentiation, or secretion. The central workflow is comparison of cellular responses within a reproducible epithelial system. The overview does not specify exact culture parameters, so those details must be established from the study protocol.
They can provide an initial in vitro indication of how a candidate treatment affects hormone-responsive endometrial biology, including gene expression, differentiation, or secretion. Such results may help prioritize therapies for further study in reproductive disease or implantation-related research. However, cell-line findings require comparison with primary cells and in vivo models before broader biological conclusions are drawn.