Zebrafish Cancer Model
Zebrafish Cancer Model - Related Videos
03:21
Zebrafish Patient-Derived Xenograft (zPDX): A Cancer Model
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2023
This video describes the technique to create a zebrafish patient derived xenograft model. In the sample protocol, we assess the efficiency of the drugs Gemcitabine and Navitoclax on the pancreatic tumor xenograft model.
09:22
Invasive Behavior of Human Breast Cancer Cells in Embryonic Zebrafish
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Cited by 44 •
2017
Here, we describe xenograft zebrafish models using two different injection sites, i.e., perivitelline space and duct of Cuvier, to investigate the invasive behavior and to assess the intravasation and extravasation potential of human breast cancer cells, respectively.
07:31
Testing the Vascular Invasive Ability of Cancer Cells in Zebrafish (Danio Rerio)
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Cited by 19 •
2016
This method utilizes zebrafish embryos to efficiently test the vascular invasive ability of cancer cells. Fluorescent cancer cells are injected into the precardiac sinus or yolk sac of developing embryos. Cancer cell vascular invasion and extravasation is assessed via fluorescence microscopy of the tail region 24 to 96 hr later.
06:14
Automating Tumor Implantation in Zebrafish Larvae for Cancer Research and Medicine
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2025
Here, we present a detailed protocol for automated tumor xenograft implantation into zebrafish larvae using a state-of-the-art robotic injection system. Furthermore, we present injection videos and validation data demonstrating the system's successful performance.
07:58
Nephrotoxin Microinjection in Zebrafish to Model Acute Kidney Injury
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Cited by 8 •
2016
Renal injuries incurred from nephrotoxins, which include drugs ranging from antibiotics to chemotherapeutics, can result in complex disorders whose pathogenesis remains incompletely understood. This protocol demonstrates how zebrafish can be used for disease modeling of these conditions, which can be applied to the identification of renoprotective measures.
FAQs
Different experimental routes reveal different stages of cancer biology. Activating oncogenes can test how cancer-promoting genetic signals contribute to tumor initiation, whereas disrupting tumor-suppressor genes examines the loss of protective controls. Chemical induction provides another way to generate tumors, and transplantation permits observation of human cancer cells in the zebrafish environment. Together, these approaches connect molecular changes with tumor behavior.
Optical transparency allows researchers to monitor tumor-associated cellular behavior directly in living embryos. This visibility is especially useful when examining processes such as angiogenesis, invasion, and metastasis, because investigators can follow cancer-related changes in an intact experimental organism. The result is a clearer connection between observable cellular behavior and broader patterns of tumor progression.
These approaches address different experimental questions. Genetic activation or disruption tests the contribution of specific oncogenes or tumor-suppressor genes, while chemical induction examines tumor formation after exposure to a tumor-inducing compound. Transplantation adds human cancer cells to the system, supporting investigation of their growth and behavior in a living vertebrate context.
Researchers first choose whether to activate oncogenes, disrupt tumor-suppressor genes, induce tumors chemically, or transplant human cancer cells. They then monitor tumor growth and cellular behavior, often using embryo transparency, and evaluate outcomes such as angiogenesis, invasion, metastasis, or treatment response. This sequence connects model setup with measurable cancer-related endpoints.
Depending on the model design, investigators can assess tumor growth, cellular behavior, angiogenesis, invasion, metastasis, and response to treatment. These endpoints allow the same broad experimental system to address both disease mechanisms and therapeutic effects. Monitoring several outcomes helps relate a molecular manipulation or transplanted cell population to visible changes in cancer progression.
Zebrafish are particularly useful when a study requires a vertebrate setting together with relatively rapid, large-scale experimentation. Their genetic and physiological similarities to humans help researchers connect molecular mechanisms to cancer biology, while the model's experimental tractability supports therapeutic discovery. These features also make it relevant to personalized cancer research, where treatment responses are important.