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Testing the Vascular Invasive Ability of Cancer Cells in Zebrafish (Danio Rerio)
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Chapters
- 00:05Title
- 00:40Injecting Cancer Cells into the Pre-cardiac Sinus of Zebrafish Embryos
- 03:58Scoring Extravasation
- 04:37Mounting Embryos onto Slides and Subsequent Fluorescence Imaging
- 05:34Results: Vascular Invasion of Cancer Cell Lines in Zebrafish Embryos
- 06:50Conclusion
This method utilizes zebrafish embryos to efficiently test the vascular invasive ability of cancer cells. Fluorescent cancer cells are injected into the precardiac sinus or yolk sac of developing embryos. Cancer cell vascular invasion and extravasation is assessed via fluorescence microscopy of the tail region 24 to 96 hr later.
