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JoVE Journal
Cancer Research

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Testing the Vascular Invasive Ability of Cancer Cells in Zebrafish (Danio Rerio)
 

Testing the Vascular Invasive Ability of Cancer Cells in Zebrafish (Danio Rerio)

Article doi: 10.3791/55007
November 3rd, 2016

Summary November 3rd, 2016

This method utilizes zebrafish embryos to efficiently test the vascular invasive ability of cancer cells. Fluorescent cancer cells are injected into the precardiac sinus or yolk sac of developing embryos. Cancer cell vascular invasion and extravasation is assessed via fluorescence microscopy of the tail region 24 to 96 hr later.

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