Amphotericin-b Perforated Patch-clamp
Amphotericin-b Perforated Patch-clamp - Related Videos
04:20
Amphotericin-B Mediated Perforated Patch-Clamp Analysis: An Electrophysiological Technique to Study Ionic Currents in Urinary Bladder Cells
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2023
This video demonstrates the characterization of cationic currents from transient receptor potential melastatin type 4 (TRPM4) ion channels in detrusor smooth muscle cells using amphotericin-B mediated perforated patch-clamp technique. The technique helps evaluate the biophysical and pharmacological properties of ion channels.
02:50
Perforated Patch-Clamp Recordings of Inner Ear Sensory Neuron Cell Bodies
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2025
This video demonstrates the patch-clamp technique for measuring ionic currents in inner ear sensory neuron cell bodies. It details the preparation and use of a polished patch-clamp pipette filled with an internal solution containing amphotericin B, which perforates the membrane to allow ion movement while preserving intracellular conditions. Voltage pulses are applied to trigger and record ion channel activity, providing insights into their properties and behavior.
06:22
Perforated Patch-Clamp Recording of Olfactory Sensory Neurons in Mice
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2025
This video demonstrates electrophysiological recording of olfactory sensory neurons (OSNs) in mice using a perforated patch-clamp technique. The olfactory epithelium, containing OSNs with fluorophore-tagged odor receptors, is isolated from a mouse and secured in a recording chamber. A recording pipette is clamped to the dendritic knob of an OSN, and nystatin within the pipette perforates the membrane to form electrical connectivity with the cell. Finally, another pipette is used to deliver an...
12:43
Patch Clamp Recordings in Inner Ear Hair Cells Isolated from Zebrafish
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Cited by 9 •
2012
The purpose of this video is to demonstrate procedures for obtaining healthy, intact hair cells from the inner ear organs of adult zebrafish and then using them for patch clamp studies aimed at characterizing the biophysical properties of their voltage-gated channels.
09:43
Patch Clamp Electrophysiology
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2023
Neuron cell membranes are populated with ion channels that control the movement of charge into and out of the cell, thereby regulating neuron firing. One extremely useful technique for investigating the biophysical properties of these channels is called patch clamp recording. In this method, neuroscientists place a polished glass micropipette against a cell and apply suction to form a high resistance seal. This process isolates a small “patch” of membrane that contains one or more ion channels.
FAQs
Amphotericin B creates small pores in the patch membrane rather than requiring the cell to be ruptured. This distinction limits extensive dilution of the cytoplasm, so recorded ionic currents arise under conditions closer to the cell’s native intracellular state. That preservation is especially useful when intracellular composition influences membrane function.
Voltage control sets defined electrical conditions while the researcher records ionic currents. Comparing channel responses under those controlled conditions helps separate changes in channel behavior from uncontrolled shifts in membrane voltage. The same recordings can also characterize membrane potential, providing complementary information about how ion transport shapes electrical activity.
Maintaining much of the native cytoplasm can help retain cellular conditions relevant to altered ion transport in cancer cells. This matters because ion-channel activity and membrane potential are linked to signaling, proliferation, migration, and sensitivity to therapeutic compounds. The technique therefore connects membrane-level measurements with cancer-related cellular responses without relying solely on disrupted intracellular conditions.
A typical workflow establishes a patch configuration, uses amphotericin B to create small membrane pores, applies voltage control, and records ionic currents under defined conditions. Researchers can then examine channel activity or membrane potential before and after pharmacological treatment. The sequence preserves intracellular contents more effectively than a recording that ruptures the cell.
The recordings can show how pharmacological compounds alter ion-channel activity, ionic currents, or membrane potential. Researchers can compare responses under controlled voltage conditions to determine whether a treatment changes channel behavior or broader membrane function. In cancer research, these outcomes help assess how altered ion transport may relate to therapeutic sensitivity.
By linking channel activity and membrane potential to cellular responses, measurements can help investigate ion transport as part of cancer-related signaling, proliferation, and migration. They can also reveal whether pharmacological compounds modify membrane function in ways associated with treatment response. Thus, the method provides electrophysiological evidence that complements broader studies of cancer-cell behavior.