Cell Extension Quantification
Cell Extension Quantification - Related Videos
09:51
Measuring Plant Cell Wall Extension (Creep) Induced by Acidic pH and by Alpha-Expansin
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Cited by 43 •
2009
We demonstrate the use of a constant-force extensometer to measure long-term extension (creep) of plant cell wall specimens induced by acidic buffers and expansin protein.
03:32
Using a Macrophage Conditioned Medium to Promote Neuron Extensions
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2025
This video demonstrates an assay for promoting the growth of neuron projections known as neurite outgrowth using the macrophage-conditioned medium. Co-culture neurons and macrophages with cAMP and harvest the macrophage-derived conditioned medium. Growing the neurons with the conditioned medium promotes neurite outgrowths. These outgrowths are fluorescently labeled and visualized using a fluorescence microscope.
28:40
Proboscis Extension Response (PER) Assay in Drosophila
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Cited by 120 •
2007
Proboscis extension response or PER is a taste behavior assay that has been used in flies as well as in honeybees. When the proboscis makes contact with an attractive substance, the fly extends its proboscis to consume the substance. Solutions of various sugars are very attractive to the fly.
15:28
Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources
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Cited by 41 •
2009
We present a method of targeted ancient DNA sequence retrieval, which we used to reconstruct the complete mitochondrial genomes of five Neandertal individuals. Comparison of these sequences with present day humans suggests that Neandertals had a long term low effective population size.
10:37
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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Cited by 15 •
2017
We present a protocol to accurately quantitate proteins with isobaric labelling, extensive fractionation, bioinformatics tools, and quality control steps in combination with liquid chromatography interfaced to a high-resolution mass spectrometer.
FAQs
Extension number indicates how frequently protrusions appear, whereas length and width describe their physical dimensions. Area captures the amount of membrane involved, orientation shows the direction of projection, and perimeter proportion indicates how extensively the cell boundary participates. Considering these features together gives a more complete view than relying on a single measurement.
Perimeter proportion places individual extensions in the context of the whole cell boundary. Two cells may have similar extension counts but differ in how much of their perimeter participates in protrusion formation. This measure therefore helps distinguish localized remodeling from broader changes in cell shape and supports comparisons across tumor cell types or experimental conditions.
A single image records the extensions present at one time, while time-lapse imaging follows their formation and retraction. This temporal information helps determine whether a treatment or genetic change affects the appearance, persistence, or loss of protrusions. Such dynamics provide additional context for interpreting cytoskeletal remodeling and motility-related behavior.
Analysis begins by identifying the cell boundary in microscopy images, followed by detecting or measuring protrusions projecting from that boundary. The workflow can then calculate number, length, area, width, orientation, and perimeter involvement. For time-lapse datasets, the same features can be tracked across images to assess extension formation and retraction.
Researchers can compare extension patterns among tumor cell types or between experimental conditions to evaluate behaviors associated with migration and invasion. The measurements can also test whether genetic changes, extracellular matrix properties, or candidate drugs alter protrusive activity. These comparisons provide objective indicators of cytoskeletal remodeling linked to metastatic spread.
The analysis can show whether a candidate drug changes extension number, dimensions, orientation, or the fraction of the perimeter involved. When combined with time-lapse imaging, it can also indicate altered formation or retraction. Comparing treated and untreated conditions helps connect these measurable changes with differences in motility- and invasion-related behavior.