Macrophage Engraftment
Macrophage Engraftment - Related Videos
04:36
Adenocarcinoma Engraftment: A Method to Implant Cancer Cells into the Murine Lungs
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2023
This video describes a method to engraft lung adenocarcinoma cells in murine lungs by preconditioning the airways with fibrosis-inducing agent–bleomycin. This method significantly enhances orthotopic engraftment of lung cancer cells into murine...
10:07
Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages
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Cited by 326 •
2013
The article describes a readily easy adaptive in vitro model to investigate macrophage polarization. In the presence of GM-CSF/M-CSF, hematopoietic stem/progenitor cells from the bone marrow are directed into monocytic differentiation, followed by M1 or M2 stimulation. The activation status can be tracked by changes in cell surface antigens, gene expression and cell signaling pathways.
10:52
Tumor Engraftment in a Xenograft Mouse Model of Human Mantle Cell Lymphoma
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Cited by 1 •
2018
Mantle cell lymphoma (MCL) is a difficult to treat B cell disorder and it is equally difficult to establish a xenograft mouse model of primary MCL to study and develop therapeutics. Here, we describe the successful establishment of MCL xenografts in mice to help understand its underlying biology.
08:15
Tracking Dynamics of Muscle Engraftment in Small Animals by In Vivo Fluorescent Imaging
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Cited by 4 •
2009
We describe an in vivo fluorescence imaging protocol to monitor muscle regeneration by GFP-labeled myoblasts after transplantation into skeletal muscles of both healthy and dystrophic mice. This protocol can be adapted to study muscle regeneration by transplantation of other types of cells and in other muscular conditions as well.
06:46
Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages
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Cited by 58 •
2016
A protocol is presented for cell culture of macrophage colony-stimulating factor (M-CSF) differentiated human monocyte-derived macrophages. The protocol utilizes cryopreservation of monocytes coupled with their bulk differentiation into macrophages. Then harvested macrophages can then be seeded into culture wells at required cell densities for carrying out experiments.
FAQs
After macrophage engraftment, the transferred cells encounter local cells and extracellular matrix in the target tissue. These interactions provide the immediate context in which the cells can persist, proliferate, or adopt tissue-specific functional states. Examining this context helps researchers connect the physical tumor microenvironment with changes in macrophage behavior rather than treating the cells as functionally isolated.
Persistence is not a single outcome. Macrophages may survive, proliferate, or change functional state after reaching the target tissue, so studies should distinguish simple presence from continued cellular activity. This distinction matters in cancer models because a population that remains detectable may have different effects on tumor growth, immune suppression, metastasis, or therapy response depending on its state.
Transferred macrophages provide a way to examine how introduced cells behave in a tissue, whereas engineered macrophages add a deliberate modification to that experimental system. The engineering component can support genetic manipulation, targeted delivery studies, or attempts to reprogram tumor-associated macrophages. Comparing these formats helps separate effects of cell engraftment from effects associated with the engineered function.
Within cancer research, the key mechanistic question is how engrafted macrophages alter the tumor environment. Their presence can be examined in relation to tumor growth, immune suppression, metastasis, and response to therapy. This links macrophage behavior to clinically relevant cancer processes and allows researchers to investigate whether changing macrophage activity produces a more antitumor environment.
A study typically begins by administering transferred or engineered macrophages to a selected target tissue. Researchers then examine whether the cells migrate into that site and interact with its local cells and extracellular matrix. Subsequent analysis can address persistence, survival, proliferation, and functional state, creating a sequence from delivery through tissue response.
It can reveal how introduced macrophages relate to tumor growth, immune suppression, metastasis, and treatment response. The approach also supports studies of targeted delivery, because macrophages can serve as vehicles within the experimental system. These outcomes help connect macrophage migration and function with broader changes in the tumor and its response to intervention.
Reprogramming strategies aim to shift tumor-associated macrophages toward antitumor activity, making engrafted cells useful for testing immunomodulatory treatments. Genetic engineering can provide a way to study such purposeful changes, while persistence in tissue allows researchers to examine their relationship to tumor growth and therapy response. This supports development of cell-based and immunomodulatory approaches.