Ion Trap Mass Spectrometer
Ion Trap Mass Spectrometer - Related Videos
11:45
Experimental Methods for Trapping Ions Using Microfabricated Surface Ion Traps
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Cited by 19 •
2017
This paper presents a microfabrication methodology for surface ion traps, as well as a detailed experimental procedure for trapping ytterbium ions in a room-temperature environment.
01:16
Mass Spectrometers
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2024
This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions: Conversion of the gas-phase analyte atoms/molecules into a beam of positive or negative charged ions by ionization. Separation of the charged species based on their mass-to-charge ratio. Recording the relative abundance of each type of ion. In the ionization chamber of...
09:38
Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
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Cited by 3 •
2013
Dithranol (DT; 1,8-dihydroxy-9,10-dihydroanthracen-9-one) has previously been reported as a MALDI matrix for tissue imaging of small molecules; protocols for the use of DT for the MALDI imaging of endogenous lipids on the surface of tissue sections by positive-ion MALDI-MS on an ultrahigh-resolution quadrupole-FTICR instrument are provided here.
08:40
Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
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Cited by 2 •
2022
Ion mobility spectrometry (IMS) is an interesting complement to mass spectrometry for the characterization of biomolecules, notably because it is sensitive to isomerism. This protocol describes a tandem IMS (IMS/IMS) experiment, which allows the isolation of a molecule and the generation of the mobility profiles of its fragments.
10:22
In Situ SIMS and IR Spectroscopy of Well-defined Surfaces Prepared by Soft Landing of Mass-selected Ions
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Cited by 4 •
2014
Soft landing of mass-selected ions onto surfaces is a powerful approach for the highly-controlled preparation of novel materials. Coupled with analysis by in situ secondary ion mass spectrometry (SIMS) and infrared reflection absorption spectroscopy (IRRAS), soft landing provides unprecedented insights into the interactions of well-defined species with surfaces.
FAQs
The radiofrequency electric fields create a confined region in which gas-phase ions remain stored rather than immediately traveling through the instrument. By changing the applied fields, the instrument makes ions unstable at selected mass-to-charge ratios and ejects them. This controlled transition from confinement to ejection allows the system to distinguish ions according to their mass-to-charge values.
Tandem operation adds a selective structural step after ion storage. An ion of interest can be isolated within the trap and then fragmented, producing information about how that ion breaks apart. The resulting fragment pattern helps characterize the original compound, making the approach more informative than measuring the intact ion alone when molecular structure is the primary question.
Mass-to-charge ratio provides the basis for separating the stored ions during controlled ejection. Ions associated with different values become unstable under different applied-field conditions, so the instrument can examine them selectively. These measurements support compound identification by revealing which ion signals are present, while tandem fragmentation adds structural information for more detailed characterization.
A typical sequence begins with gas-phase ions entering the trapping region, where the applied radiofrequency fields confine them. The fields are then varied so ions become unstable and leave the trap according to their mass-to-charge ratios. When structural information is needed, the sequence can include isolation of a selected ion followed by fragmentation before measurement.
It is useful when a sample contains multiple compounds that require both detection and characterization. The platform can provide qualitative information for identifying components and quantitative information for measuring them. Its ability to selectively examine ions and, when needed, fragment them makes it relevant to complex mixtures such as pharmaceutical, metabolite, and environmental samples.
In these areas, researchers may need to determine which compounds occur in a complex mixture and obtain information about their molecular structures. Ion storage enables selective examination of measured species, while tandem isolation and fragmentation can strengthen compound characterization. The same platform therefore supports chemical analysis across pharmaceutical, metabolite, and environmental research contexts.