Cardiac Tissue Isolation
Cardiac Tissue Isolation - Related Videos
08:15
Isolation of Perivascular Multipotent Precursor Cell Populations from Human Cardiac Tissue
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Cited by 7 •
2016
Human cardiac tissue harbours multipotent perivascular precursor cell populations that may be suitable for myocardial regeneration. The technique described here allows for the simultaneous isolation and purification of two multipotent stromal cell populations associated with native blood vessels, i.e. CD146+CD34- pericytes and CD34+CD146- adventitial cells, from the human myocardium.
07:03
Robust Mitochondrial Isolation from Rodent Cardiac Tissue
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Cited by 1 •
2024
Bioenergetic and metabolomic studies on mitochondria have revealed their multifaceted role in many diseases, but the isolation methods for these organelles vary. The method detailed here is capable of purifying high-quality mitochondria from multiple tissue sources. Quality is determined by respiratory control ratios and other metrics assessed with high-resolution respirometry.
04:40
Creation of Cardiac Tissue Exhibiting Mechanical Integration of Spheroids Using 3D Bioprinting
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Cited by 55 •
2017
This protocol describes 3D bioprinting of cardiac tissue without the use of biomaterials. 3D bioprinted cardiac patches exhibit mechanical integration of component spheroids and are highly promising in cardiac tissue regeneration and as 3D models of heart disease.
07:26
Isolation of Functional Cardiac Immune Cells
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Cited by 9 •
2011
This method for isolating functional immune cells from the heart provides an alternative to the conventional methods of collagenase digestion, which causes unwanted immune cell activation, resulting in a decreased responsiveness of these cells. Our method of isolation yields functional cardiac immune cells by avoiding problems associated with enzymatic digestion.
10:09
Capillary Force Lithography for Cardiac Tissue Engineering
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Cited by 27 •
2014
In this protocol, we demonstrate the fabrication of biomimetic cardiac cell culture substrata made from two distinct polymeric materials using capillary force lithography. The described methods provide a scalable, cost-effective technique to engineer the structure and function of macroscopic cardiac tissues for in vitro and in vivo applications.
FAQs
The procedure must balance two competing goals: disrupting extracellular connections enough to produce usable cells or tissue fragments, while limiting damage that could reduce viability or alter developmental characteristics. Careful dissection establishes the desired tissue boundaries, and controlled mechanical or enzymatic dissociation then separates the material. This balance determines whether the preparation is suitable for structural, functional, or developmental analysis.
Mechanical dissociation separates cardiac material through physical disruption, whereas enzymatic dissociation helps disrupt extracellular connections through biochemical activity. Both approaches support the generation of viable cells or tissue fragments, but they provide different ways to control how thoroughly the tissue is separated. Selecting between them depends on the preparation required for downstream imaging, culture, molecular assays, or tissue analysis.
Cardiac tissue changes as the heart forms and matures, so isolation must preserve information about the developmental state of the sample. Maintaining viable tissue fragments or cells allows researchers to examine cardiomyocyte differentiation, tissue organization, gene expression, and cell signaling in relation to developmental stage. Comparisons across stages can therefore connect cellular observations with broader patterns of heart development.
A typical workflow begins with careful dissection of the heart tissue, followed by mechanical or enzymatic dissociation to disrupt extracellular connections. The resulting preparation is then used as viable cells or tissue fragments for a selected analysis. Controlled and sterile conditions are maintained throughout because the quality of the isolated material affects its suitability for imaging, culture, molecular assays, and developmental comparisons.
Controlled conditions help maintain the intended physical state and viability of the isolated preparation, while sterile handling supports its use in culture and other downstream analyses. These requirements are especially important when researchers need to preserve developmental characteristics rather than simply obtain separated material. Consistent handling also makes comparisons among normal, perturbed, and differently staged cardiac tissues more interpretable.
Isolated cardiac preparations provide material for imaging, culture, and molecular assays that examine how heart tissues form and mature. Researchers can investigate cardiomyocyte differentiation, tissue organization, gene expression, and cell signaling, then compare normal development with perturbed conditions. These applications connect structural observations with cellular and molecular changes across developmental stages, helping clarify mechanisms of cardiac development.