In this protocol, we demonstrate the fabrication of biomimetic cardiac cell culture substrata made from two distinct polymeric materials using capillary force lithography. The described methods provide a scalable, cost-effective technique to engineer the structure and function of macroscopic cardiac tissues for in vitro and in vivo applications.
Macadangdang, J., Lee, H. J., Carson, D., Jiao, A., Fugate, J., Pabon, L., Regnier, M., Murry, C., Kim, D. Capillary Force Lithography for Cardiac Tissue Engineering. J. Vis. Exp. (88), e50039, doi:10.3791/50039 (2014).