Embryo Collection Cages

Embryo collection cages are laboratory enclosures that house breeding animals and direct the collection of newly laid embryos for developmental biology research. In a typical setup, adults are confined above a removable collection surface, such as an agar-based plate, where they deposit eggs; researchers exchange the surface at defined intervals to obtain age-matched embryos. This controlled timing supports studies of embryogenesis, gene function, cell differentiation, and tissue formation by producing synchronized samples for microscopy, molecular analysis, or experimental manipulation. Collection cages also improve sample handling and help standardize developmental comparisons across experiments.

Embryo Collection Cages - Related Videos

Research

JoVE Journal - Biology
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Maintenance of a Drosophila melanogaster Population Cage

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Cited by 8 •

2016

This manuscript reports a detailed protocol for culturing, on a regular basis, a population of Drosophila melanogaster using a fly population cage.

Research

JoVE Journal - Biology

Live Dissection of Drosophila Embryos: Streamlined Methods for Screening Mutant Collections by Antibody Staining

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Cited by 30 •

2009

We describe a streamlined protocol for generating "fillet" preparations of Drosophila embryos of specific genotypes. This protocol allows efficient execution of a variety of genetic screens. It also allows excellent visualization of structures in the late embryo.

Collection and Cryopreservation of Hamster Oocytes and Mouse Embryos

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Cited by 9 •

2009

In this video-article we present a step-by-step demonstration on how to collect and cryopreserve hamster oocytes with high post-thaw survival rates. The same procedure can also be applied to successfully freeze and thaw mouse embryos at different stages of preimplantation development.

Microinjection of Live Drosophila Embryos: Early Delivery of Reagents to the Developing Embryo

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2023

This video describes how to perform microinjections that deliver reagents into live Drosophila embryos. Researchers can use this method to introduce exogenous compounds like nucleic acids and proteins during the early stages of embryo development. The featured protocol demonstrates how to set up and perform the procedure for injection of any soluble reagent–here, a fluorescent protein for live imaging experiments.

Education

JoVE Core - Anatomy and Physiology

The Thoracic Cage: Sternum

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2023

The thoracic or rib cage forms the body's thorax (chest) portion. Its primary function in the body is to protect vital organs in the thoracic cavity, such as the heart and the lungs. It consists of 12 pairs of ribs with their costal cartilages and the sternum. The ribs are anchored posteriorly to the 12 thoracic vertebrae (T1-T12). The sternum is the elongated bony structure on the anterior side of the thoracic cage. It consists of three parts: the manubrium, the body, and the xiphoid process.

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