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Collection and Cryopreservation of Hamster Oocytes and Mouse Embryos
JoVE Journal
Biology
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JoVE Journal Biology
Collection and Cryopreservation of Hamster Oocytes and Mouse Embryos
DOI:

13:36 min

March 27, 2009

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Chapters

  • 00:00Title
  • 00:15Oocyte Collection
  • 03:49Preparation of freezing and thawing solutions
  • 06:32Freezing protocol
  • 11:39Thawing protocol
  • 13:18Representative results

Summary

Automatic Translation

In this video-article we present a step-by-step demonstration on how to collect and cryopreserve hamster oocytes with high post-thaw survival rates. The same procedure can also be applied to successfully freeze and thaw mouse embryos at different stages of preimplantation development.

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