Hemogenic Intermediate Cells
Hemogenic Intermediate Cells - Related Videos
08:56
Isolation of Murine Embryonic Hemogenic Endothelial Cells
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Cited by 11 •
2016
Hematopoietic stem and progenitor cells (HSPC) derive from specialized (hemogenic) endothelial cells during development, yet little is known about the process by which some endothelial cells specify to become blood forming. We demonstrate a flow-cytometry based method allowing simultaneous isolation of hemogenic endothelial cells and HSPC from murine embryonic tissues.
11:00
Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program
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Cited by 1 •
2016
The protocol described here details the induction of a hemogenic program in mouse embryonic fibroblasts via overexpression of a minimal set of transcription factors. This technology may be translated to the human system to provide platforms for future study of hematopoiesis, hematologic disease, and hematopoietic stem cell transplant.
04:23
Directed Differentiation of Hemogenic Endothelial Cells from Human Pluripotent Stem Cells
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Cited by 5 •
2021
Presented here is a simple protocol for the directed differentiation of hemogenic endothelial cells from human pluripotent stem cells in approximately 1 week.
10:32
Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model
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Cited by 21 •
2014
Mouse embryonic fibroblast can be reprogrammed into induced pluripotent stem cells at low efficiency by the forced expression of transcription factors Oct-4, Sox-2, Klf-4, c-Myc. The rare intermediates of the reprogramming reaction are FACS isolated via labeling with antibodies against cell surface makers Thy-1.2, Ssea-1, and Epcam.
00:57
Formation of Intermediate Filaments
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2023
Intermediate filaments are cytoskeletal proteins with higher tensile strength and flexibility than microfilaments and microtubules. Unlike the other two cytoskeletal proteins, intermediate filament formation lacks the enzymatic activity to hydrolyze nucleotides like ATP and GTP to generate energy for polymerization. Therefore, the formation of intermediate filaments is multistep self-assembly. The involvement of any accessory proteins in intermediate filament formation has not yet been reported.
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During the endothelial-to-hematopoietic transition, coordinated changes in cell shape and adhesion help these cells move away from an endothelial state, while lineage-specific gene expression supports hematopoietic identity. Examining these linked changes allows developmental biologists to connect visible cellular behavior with the molecular decisions that produce nascent blood-forming cells.
Their temporary existence marks a narrow developmental interval between vascular formation and blood production. Because they occupy this intermediate state, they can reveal when endothelial cells acquire hematopoietic potential and which regulatory events accompany that shift. This makes them useful for investigating cell-fate decisions that may be difficult to observe once definitive hematopoiesis is established.
They help clarify how a vascular-associated endothelial population gives rise to hematopoietic cells through an endothelial-to-hematopoietic transition. The process links two major developmental programs rather than treating blood formation as an isolated event. Studying this connection is particularly relevant to understanding how embryonic tissues coordinate vessel development with the emergence of blood-forming potential.
In developing embryos, these intermediates contribute to intra-aortic hematopoietic clusters, providing a developmental connection between the transitional cells and an organized site of early blood production. Their association with these clusters helps researchers examine how nascent hematopoietic cells emerge in the embryo and how this early activity contributes to the establishment of definitive hematopoiesis.
Investigating these cells can identify regulators that influence endothelial-to-hematopoietic cell-fate decisions. Such regulators may help explain why some embryonic endothelial cells acquire blood-forming potential while others remain associated with vascular development. This information strengthens developmental models of hematopoiesis and provides a basis for evaluating strategies intended to generate hematopoietic stem and progenitor cells.
The transition represented by Hemogenic Intermediate Cells offers a developmental framework for efforts to produce functional hematopoietic stem and progenitor cells. By examining the associated cellular and gene-expression changes, researchers can focus on stages that may be important for acquiring hematopoietic identity. The broader goal is to improve the developmental fidelity of stem and progenitor cell generation.
These cells connect embryonic blood development with research applications that require a better understanding of hematopoietic cell production. Their study can inform disease models by clarifying how blood-forming lineages arise and can support regenerative research aimed at generating functional hematopoietic stem and progenitor cells. Their developmental context therefore links basic biology with prospective experimental applications.