Lung Organoids
Lung Organoids - Related Videos
03:41
CRC Organoid Culture: A Method to Obtain 3D Organoids from Colorectal Cancer Cells
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2023
This video describes the detailed protocol for generating 3D organoids from murine-model derived colorectal cancer cells by propagating them on artificial extracellular matrix coated plates. These CRC organoids can be used as a model to study the primary growth of human tumor cells and metastasis.
11:49
Isolation and Enrichment of Human Lung Epithelial Progenitor Cells for Organoid Culture
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Cited by 17 •
2020
This article provides a detailed methodology for tissue dissociation and cellular fractionation approaches allowing enrichment of viable epithelial cells from proximal and distal regions of the human lung. Herein these approaches are applied for the functional analysis of lung epithelial progenitor cells through the use of 3D organoids culture models.
10:12
Establishing Human Lung Organoids and Proximal Differentiation to Generate Mature Airway Organoids
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Cited by 11 •
2022
The protocol presents a method to derive human lung organoids from primary lung tissues, expand the lung organoids and induce proximal differentiation to generate 3D and 2D airway organoids that faithfully phenocopy the human airway epithelium.
05:11
Organoid Electroporation: A Method to Transfect Plasmid DNA into Gastrointestinal Organoids
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2023
This video describes the electroporation protocol for transfecting large plasmids into three-dimensional gastrointestinal organoids to prove its universal functionality in different entities, namely pancreatic ductal adenocarcinoma (PDAC), colorectal cancer (CRC), cholangiocarcinoma (CCC), and gastric cancer (GC) organoids.
10:30
Organoids as Model for Infectious Diseases: Culture of Human and Murine Stomach Organoids and Microinjection of Helicobacter Pylori
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Cited by 144 •
2015
Stem cell derived cultures harbor tremendous potential to model infectious diseases. Here, the culture of mouse and human gastric organoids derived from adult stem cells is described. The organoids are microinjected with the gastric pathogen Helicobacter pylori.
FAQs
Defined biochemical signals guide stem or progenitor cells toward particular respiratory lineages and developmental states. Their effects depend on how cells interpret these cues within a supportive extracellular matrix, which provides a three-dimensional environment for organization. By adjusting signaling conditions, researchers can examine how lineage specification and epithelial maturation are regulated during the formation of airway or alveolar-like tissue.
The extracellular matrix supplies a structural environment that allows developing cells to organize in three dimensions rather than spreading as a simple layer. Within this support, cells establish spatial relationships that resemble aspects of developing respiratory tissue. This organization makes it possible to investigate tissue architecture, epithelial maturation, and developmental processes that are difficult to reproduce in less structured culture systems.
Lung organoids provide a controlled setting for examining lineage specification, branching morphogenesis, and epithelial maturation. Lineage studies focus on how progenitor cells acquire distinct respiratory identities, whereas branching morphogenesis concerns the formation of organized tissue patterns. Researchers can follow these processes in developing airway or alveolar-like structures and relate changes in cellular identity to broader tissue organization.
Their three-dimensional organization preserves relationships among developing cell populations, allowing researchers to connect cellular decisions with tissue-level structure. Because the system is maintained in vitro, developmental conditions can be examined under controlled biochemical environments. This helps clarify how respiratory tissues form and provides a tractable model for testing developmental hypotheses without relying exclusively on animal-based studies.
A general workflow begins with stem or progenitor cells, exposes them to defined biochemical signals, and places them in a supportive extracellular matrix. The cells then self-organize into developing airway or alveolar-like tissue. Researchers can examine the resulting populations and spatial relationships to evaluate lineage specification, branching patterns, and epithelial maturation under controlled culture conditions.
These models can be applied to congenital lung disease, infectious lung disease, and drug-response studies. Their developmental features help researchers investigate how abnormal tissue formation or disease-related changes affect respiratory structures. Because conditions can be controlled in vitro, organoids also provide a platform for comparing responses across experiments and for evaluating how developing lung-like tissues react to candidate treatments.
Patient-specific organoids can connect an individual’s cellular background with respiratory tissue development and disease responses. This supports research into congenital or infectious conditions and may help evaluate drug effects in a more individualized model. Their developmental and tissue-organizing properties also make them relevant to regenerative strategies, where researchers study how respiratory tissues might be restored or replaced.