Vascularization Method
Vascularization Method - Related Videos
06:05
DiI Perfusion as a Method for Vascular Visualization in Ambystoma mexicanum
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Cited by 3 •
2017
Using a lipophilic 1,1'-Dioctadecy-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI) staining technique, Ambystoma mexicanum can undergo vascular perfusion to allow for easy visualization of the vasculature.
09:38
Scaling of Engineered Vascular Grafts Using 3D Printed Guides and the Ring Stacking Method
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Cited by 6 •
2017
Scalable engineered blood vessels would improve clinical applicability. Using easily sizable 3D-printed guides, rings of vascular smooth muscle were created and stacked into a tubular form, forming a vascular graft. Grafts can be sized to meet the range of human coronary artery dimensions by simply changing the 3D-printed guide size.
08:18
Engineered Vascularized Muscle Flap
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Cited by 6 •
2016
To date, thick tissue defects are typically reconstructed by applying autologous tissue flaps or engineered tissues. In this protocol, we present a new method for engineering vascularized tissue flap bearing an autologous pedicle, to serve as a substitute to autologous flaps.
11:10
Visualization of Vascular and Parenchymal Regeneration after 70% Partial Hepatectomy in Normal Mice
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Cited by 3 •
2016
Tools used for visualizing vascular regeneration require methods for contrasting the vascular trees. This film demonstrated a delicate injection technique used to achieve optimal contrasting of the vascular trees and illustrate the potential benefits resulting from a detailed analysis of the resulting specimen using µCT and histological serial sections.
08:22
Microfluidic Bioprinting for Engineering Vascularized Tissues and Organoids
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Cited by 37 •
2017
We provide a generalized protocol based on a microfluidic bioprinting strategy for engineering a microfibrous vascular bed, where a secondary cell type could be further seeded into the interstitial space of this microfibrous structure to generate vascularized tissues and organoids.
FAQs
The choice determines the developmental starting point of the model. Vasculogenesis is suited to systems in which endothelial progenitor cells must organize into vessels, whereas angiogenesis examines how existing vessels extend through endothelial-cell migration, proliferation, and lumen formation. This distinction helps researchers investigate different stages of vessel development and interpret how a tissue acquires its network.
These components provide different ways to guide vessel development in tissues or engineered constructs. Growth factors can regulate vascular growth, biomaterials can support the engineered environment, and co-cultured cells can contribute cellular interactions during network formation. Comparing these strategies allows researchers to examine how experimental conditions influence vascular organization, tissue maturation, and construct development.
Lumen formation indicates that endothelial cells have progressed beyond simple assembly or extension toward creating vessel structures capable of supporting transport. In a developing tissue or engineered construct, this outcome is relevant because a vascular network must help deliver oxygen and nutrients while removing waste. It also provides a morphological readout for studying vessel patterning and maturation.
Planning commonly focuses on the tissue or engineered construct, endothelial progenitor cells or existing vessels, and the guidance system used to regulate growth. Depending on the developmental question, researchers may incorporate growth factors, biomaterials, or co-cultured cells. These choices should match whether the study examines new vessel assembly, vessel extension, organ formation, or tissue maturation.
They can be used to support organoid development and to model how blood vessels participate in organ formation and tissue maturation. By examining vessel growth and patterning within these systems, researchers can study relationships between vascular networks and developing tissues. Such models provide a way to evaluate developmental processes in settings that include engineered or organoid-based constructs.
They are useful when engineered tissues require a functional blood vessel network to support continued development. Vascularization can address the transport needs of a construct by enabling oxygen and nutrient delivery and waste removal. Researchers also use these approaches to examine tissue maturation and to explore how vascular growth can be regulated in regenerative medicine applications.
Vascularization models provide systems in which researchers can observe how interventions affect vessel development, network formation, or patterning. Because the methods can involve vasculogenesis, angiogenesis, growth factors, biomaterials, or co-cultured cells, they offer multiple experimental contexts for studying regulation of vascular growth. This supports therapy evaluation in developmental and engineered-tissue settings.