Fillets
Fillets - Related Videos
03:16
Larval Fillet Preparation: A Method to Visualize Intact Sensory Neurons and Associated Epidermal Cells
0 Views •
2023
This video describes how to prepare larval fillets used to visualize sensory dendritic arborization (da) neurons and their associated epidermal cells. The featured protocol shows in detail how to remove muscle tissue from the body wall while preserving the cells' morphology, which improves immunostaining and visualization of the otherwise obscured da neurons and epidermal cells.
06:41
Removal of Drosophila Muscle Tissue from Larval Fillets for Immunofluorescence Analysis of Sensory Neurons and Epidermal Cells
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Cited by 13 •
2016
Studies of neuronal morphogenesis using Drosophila larval dendritic arborization (da) neurons benefit from in situ visualization of neuronal and epidermal proteins by immunofluorescence. We describe a procedure that improves immunofluorescence analysis of da neurons and surrounding epidermal cells by removing muscle tissue from the larval body wall.
09:42
Morphological Analysis of Drosophila Larval Peripheral Sensory Neuron Dendrites and Axons Using Genetic Mosaics
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Cited by 16 •
2011
The dendritic arborization sensory neurons of the Drosophila larval peripheral nervous system are useful models to elucidate both general and neuron class-specific mechanisms of neuron differentiation. We present a practical guide to generate and analyze dendritic arborization neuron genetic mosaics.
10:19
Three-Dimensionally Printed Microfluidic Cross-flow System for Ultrafiltration/Nanofiltration Membrane Performance Testing
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Cited by 9 •
2016
Design and fabrication of a three-dimensionally (3-D) printed microfluidic cross-flow filtration system is demonstrated. The system is used to test performance and observe fouling of ultrafiltration and nanofiltration (thin film composite) membranes.
05:07
Alternative Method of Removing Otoliths from Sturgeon
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Cited by 3 •
2016
The goal of the protocol is to show an effective method to extract the otoliths from sturgeon carcasses.
FAQs
A controlled radius changes the direction of a load path gradually instead of forcing it through an abrupt corner. This more even transition spreads applied loads across a larger region, lowering the localized stress that can initiate cracks. The effect is especially important where intersecting surfaces or features experience significant mechanical loading during service.
The radius must match how the component is loaded and how its material responds to stress. A radius that is suitable for one part may not provide the same performance in another with different loading conditions or material behavior. Engineers therefore select the size while also considering available space and manufacturing constraints.
Sharp geometric transitions can create localized stress concentrations where cracks may begin. By distributing applied loads more gradually, a fillet reduces this concentration and can lower the likelihood of crack initiation during repeated service. Its contribution to fatigue performance depends on the selected radius, the loading conditions, and the material behavior of the component.
A sharp corner creates an abrupt geometric change, concentrating applied stress at a small location. Replacing it with a controlled radius produces a smoother load transition and can reduce the conditions associated with crack initiation and fatigue failure. The rounded form may also support safer component behavior and more reliable performance when space and fabrication requirements allow it.
Design decisions should account for loading conditions, material behavior, manufacturing constraints, and available space. The selected radius must support gradual load transfer without conflicting with the way the part will be produced or the geometry available around the feature. Considering these factors together helps balance strength, fatigue resistance, manufacturability, and service reliability.
Fillets can be incorporated into machined parts, castings, welds, and structural components. Their role may extend beyond mechanical strength: suitable transitions can improve material flow during fabrication, simplify inspection, and accommodate production constraints. The appropriate design depends on the component’s geometry, material behavior, loading conditions, and the fabrication process being used.
Properly designed fillets can extend service life by reducing localized stress and the likelihood of fatigue-related crack initiation. They may also improve manufacturability, support material flow, simplify inspection, and enhance safety. These benefits apply across mechanical and structural systems when the radius is selected consistently with loading, material, fabrication constraints, and available space.