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Removal of Drosophila Muscle Tissue from Larval Fillets for Immunofluorescence Analysis of Sensory Neurons and Epidermal Cells
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Neuroscience
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JoVE Journal Neuroscience
Removal of Drosophila Muscle Tissue from Larval Fillets for Immunofluorescence Analysis of Sensory Neurons and Epidermal Cells

Removal of Drosophila Muscle Tissue from Larval Fillets for Immunofluorescence Analysis of Sensory Neurons and Epidermal Cells

DOI:
10.3791/54670-v

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06:41 min

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November 02, 2016

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,
1Department of Molecular Biology,Princeton University

Chapters

  • 00:05Title
  • 00:41Preparation and Dissection of Larvae
  • 02:34Fixation and Mounting of Larval Fillets
  • 04:10Results: Muscle Removal Enables Enhanced Imaging of Da Neurons and the Epidermis
  • 06:02Conclusion

Summary

Automatic Translation

Automatic Translation

Studies of neuronal morphogenesis using Drosophila larval dendritic arborization (da) neurons benefit from in situ visualization of neuronal and epidermal proteins by immunofluorescence. We describe a procedure that improves immunofluorescence analysis of da neurons and surrounding epidermal cells by removing muscle tissue from the larval body wall.

Tags

Keywords: DrosophilaImmunofluorescenceLarval FilletsDendritic ArborizationDa NeuronsEpidermal CellsDissectionFixationFormaldehydePBS

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