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Removal of Drosophila Muscle Tissue from Larval Fillets for Immunofluorescence Analysis of Sensory Neurons and Epidermal Cells

Article DOI: 10.3791/54670-v • 06:41 min • November 2nd, 2016
November 2nd, 2016 •
1, 1
1Department of Molecular Biology, Princeton University

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Automatic Translation

Studies of neuronal morphogenesis using Drosophila larval dendritic arborization (da) neurons benefit from in situ visualization of neuronal and epidermal proteins by immunofluorescence. We describe a procedure that improves immunofluorescence analysis of da neurons and surrounding epidermal cells by removing muscle tissue from the larval body wall.

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Drosophila Muscle Tissue Removal Larval Fillets Immunofluorescence Analysis Sensory Neurons Epidermal Cells Dendritic Arborization Da Neurons Muscle Tissue Removal Technique Super Resolution Microscopy Cold Saline Silicone Elastomer Dish Stereo Microscope Ventral Side Up Abdominal Denticle Belts Fine Dissecting Scissors Internal Organs Removal CNS Removal Gut Removal Trachea Removal
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