Cdna Conversion
Cdna Conversion - Related Videos
09:44
Rapid Amplification of cDNA Ends
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2023
Source: Pablo Sanchez Bosch2, Sean Corcoran2 and Katja Brückner1,2,3 1Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research 2Department of Cell and Tissue Biology, 3Cardiovascular Research Institute, University of California San Francisco, San Francisco, CA, USA Rapid Amplification of cDNA Ends (RACE) is a technique that allows amplification of full-length cDNA from mRNA by extending to the 3’ or 5’ end, even without prior knowledge of the sequence (Frohman et al.,...
02:08
Gene Conversion
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2020
Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
02:08
Gene Conversion
0 Views •
2023
Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
07:01
Processing the Loblolly Pine PtGen2 cDNA Microarray
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Cited by 6 •
2009
The cDNA microarray PtGen2 was developed for gene expression studies in loblolly pine, P. taeda, and other conifer species. Here, we show pre- and post-hybridization handling and washing techniques that can be used with this array to yield better consistency, reduced artifacts, and lower backgrounds.
15:21
RNA Isolation from Embryonic Zebrafish and cDNA Synthesis for Gene Expression Analysis
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Cited by 111 •
2009
The isolation of high quality, intact RNA is an essential step in many laboratory protocols. Here, we demonstrate RNA extraction from whole zebrafish embryos and cDNA synthesis for subsequent application in various experimental procedures including gene expression microarray analysis.
FAQs
Reverse transcriptase reads an RNA strand as a template and links deoxyribonucleotides into a complementary DNA strand. Primers provide the starting point required for DNA synthesis, allowing the enzyme to copy RNA-derived information into a DNA form. This enzyme-template relationship is central to using RNA measurements with analytical methods designed for DNA.
Primers initiate DNA synthesis by providing reverse transcriptase with a starting point on the RNA template. Without this initiation step, the enzyme cannot begin building the complementary strand. Primer-dependent synthesis therefore determines whether RNA information can be transferred into cDNA for later procedures such as reverse-transcription PCR, expression analysis, or transcript profiling.
The resulting cDNA represents expressed transcripts, whereas genomic DNA represents the broader genetic material rather than only the RNA molecules present in a cell. This distinction makes cDNA useful for examining which genes are active under particular cellular conditions. It also supports comparisons of gene regulation between different cellular states.
Because the cDNA originates from RNA, its sequence reflects transcript information available in the analyzed sample rather than the entire genome. The result therefore provides a view of gene expression at that cellular state. Interpreting cDNA data requires recognizing that changes may reflect differences in expressed transcripts and regulatory activity, not simply differences in genomic content.
A basic workflow begins with an RNA template, adds primers to initiate synthesis, and uses reverse transcriptase to incorporate deoxyribonucleotides into a complementary DNA strand. The resulting cDNA can then serve as material for DNA-focused analyses. In practice, this sequence connects RNA collection with downstream reverse-transcription PCR, quantitative expression analysis, profiling, or sequencing-library preparation.
Researchers use the technique when they need to analyze RNA-based genetic information with DNA-focused methods. It supports reverse-transcription PCR, quantitative gene-expression analysis, transcript profiling, and preparation of libraries for sequencing. These applications help investigate expressed genes, compare cellular states, and study how gene regulation changes across biological conditions.