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Chapters
- 00:00Title
- 00:43Microarray Slide Pre-Wash
- 01:05Pre-Hybridization
- 01:31Post Pre-Hybridization
- 03:27Post-Hybridization Wash
- 05:56Imaging
- 06:39Conclusion
The cDNA microarray PtGen2 was developed for gene expression studies in loblolly pine, P. taeda, and other conifer species. Here, we show pre- and post-hybridization handling and washing techniques that can be used with this array to yield better consistency, reduced artifacts, and lower backgrounds.
Tags
Loblolly Pine PtGen2CDNA MicroarrayGene ExpressionConifer SpeciesGene DiscoveryNeedleStemCy-dye Labeled Conifer Target CDNAsAmplified And Non-amplified Indirect Labeling MethodsHybridizationWashing ConditionsHandling SlidesPre-hybridizationHybridization ProceduresTarget CDNA GenerationLabeling And Cleanup ProtocolsSoftware For Data ProcessingProprietary Print BufferSDS SolutionSalt RemovalLifterSlips™Labeled CDNA Loading
