Dna Hybridization Capture
Dna Hybridization Capture - Related Videos
06:26
Selective Capture of 5-hydroxymethylcytosine from Genomic DNA
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Cited by 5 •
2012
Described is a two-step labeling process using β-glucosyltransferase (β-GT) to transfer an azide-glucose to 5-hmC, followed by click chemistry to transfer a biotin linker for easy and density-independent enrichment. This efficient and specific labeling method enables enrichment of 5-hmC with extremely low background and high-throughput epigenomic mapping via next-generation sequencing.
16:42
Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides
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Cited by 4 •
2010
This work shows how to form an RNA/DNA hybrid at the chromosomal level and reveal transfer of genetic information from RNA to genomic DNA in yeast cells.
06:00
HCR-DNA FISH: A Fluorescence In Situ Hybridization Technique to Detect Viral DNA in Infected Cells
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2023
In this video, the cells infected with MCPyV virions were subjected to in situ hybridization chain reaction to detect MCPyV specific genomes. Further, the DNA-HCR technique was combined with FISH to visualize the MCPyV DNA in infected human skin cells.
08:40
Methyl-binding DNA capture Sequencing for Patient Tissues
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Cited by 1 •
2016
Here we present a protocol to investigate genome wide DNA methylation in large scale clinical patient screening studies using the Methyl-Binding DNA Capture sequencing (MBDCap-seq or MBD-seq) technology and the subsequent bioinformatics analysis pipeline.
11:36
Simple Method for Fluorescence DNA In Situ Hybridization to Squashed Chromosomes
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Cited by 55 •
2015
Here, we present a simple method for performing fluorescence DNA in situ hybridization (DNA ISH) to visualize repetitive heterochromatic sequences on slide-mounted chromosomes. The method requires minimal reagents and it is versatile for use with short or long probes, different tissues, and detection with fluorescence or non-fluorescence-based signals.
FAQs
Selectivity comes from sequence complementarity: a single-stranded probe can anneal to a target sequence when the two strands match under the chosen conditions. Denaturing the sample first separates its DNA strands, making target sequences accessible. Controlled reannealing then favors probe-target duplex formation, which determines which genomic fragments enter the enriched fraction.
Probe design affects how target fragments are handled during recovery. DNA hybridization capture may use labeled probes or probes that are immobilized, and either format supplies sequence-specific recognition of genomic regions. This choice is part of the capture setup, while the central requirement remains complementary pairing between the probe and the desired DNA.
Post-hybridization washing determines which DNA remains associated with the captured fraction. Nonspecific fragments are removed, whereas DNA associated with complementary probes is retained for downstream use. This cleanup is important because enrichment is valuable only if the recovered material is more focused than the starting genomic sample, reducing the amount of sequencing needed for selected regions.
After capture, the recovered DNA can be amplified before analysis. Amplification provides more material from the enriched fraction, allowing selected loci to proceed to sequencing, genotyping, or mutation detection. The order matters conceptually: enrichment narrows the genomic content first, and amplification then supports measurement of those regions rather than the entire sample.
DNA hybridization capture is useful when analysis focuses on particular genomic regions rather than unrestricted genomic sampling. In genetics, that focus can support examination of disease-associated genes, detection of mutations, investigation of structural variation, and studies of genome organization. The approach concentrates analytical effort on selected loci, making targeted projects more practical.
Enrichment can improve sensitivity for rare variants because the analysis receives a greater proportion of DNA from loci of interest than an untargeted approach would provide. It also reduces sequencing effort by limiting downstream analysis to selected regions, helping researchers focus resources on disease-associated genes or other defined genomic targets.