18,000 Views
•
11:36 min
•
January 06, 2015
DOI:
Summary
Automatically generated
Here, we present a simple method for performing fluorescence DNA in situ hybridization (DNA ISH) to visualize repetitive heterochromatic sequences on slide-mounted chromosomes. The method requires minimal reagents and it is versatile for use with short or long probes, different tissues, and detection with fluorescence or non-fluorescence-based signals.
Related Videos
13:06
Pyrosequencing: A Simple Method for Accurate Genotyping
Related Videos
27314 Views
15:42
Double Whole Mount in situ Hybridization of Early Chick Embryos
Related Videos
12357 Views
16:19
Fluorescence in situ hybridization (FISH) Protocol in Human Sperm
Related Videos
30042 Views
10:16
Detection of Viral RNA by Fluorescence in situ Hybridization (FISH)
Related Videos
38482 Views
08:48
High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization
Related Videos
11508 Views
07:00
Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae
Related Videos
34679 Views
12:21
MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues
Related Videos
14850 Views
15:54
Combined DNA-RNA Fluorescent In situ Hybridization (FISH) to Study X Chromosome Inactivation in Differentiated Female Mouse Embryonic Stem Cells
Related Videos
27611 Views
07:33
A Whole Mount In Situ Hybridization Method for the Gastropod Mollusc Lymnaea stagnalis
Related Videos
8731 Views
07:56
Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method
Related Videos
18039 Views
Read Article
Cite this Article
Larracuente, A. M., Ferree, P. M. Simple Method for Fluorescence DNA In Situ Hybridization to Squashed Chromosomes. J. Vis. Exp. (95), e52288, doi:10.3791/52288 (2015).
Copy