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Simple Method for Fluorescence DNA In Situ Hybridization to Squashed Chromosomes

Article DOI: 10.3791/52288 • 11:36 min • January 6th, 2015
January 6th, 2015 •
1, 2
1Department of Biology, University of Rochester, 2W. M. Keck Science Department, Claremont McKenna, Pitzer, and Scripps Colleges

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Here, we present a simple method for performing fluorescence DNA in situ hybridization (DNA ISH) to visualize repetitive heterochromatic sequences on slide-mounted chromosomes. The method requires minimal reagents and it is versatile for use with short or long probes, different tissues, and detection with fluorescence or non-fluorescence-based signals.

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Fluorescence DNA In Situ Hybridization Squashed Chromosomes Mapping Sequences Specific Chromosome Regions Heterochromatic Regions Computational Approaches Streamlined Protocol Formamide Washes DNA ISH Protocols Short Single Strand DNA Probes Fluorescent Dyes Repetitive DNA Sequences Heterochromatic Chromosomal Regions Insect Tissue Types Larger Probes Single Copy DNA Sequences Drosophila Melanogaster Neural Cells Nasonia Vitripennis Spermatocytes Hybridization Patterns Commercially Synthesized Probes Laboratory Supplies Reagents Investigators
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