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January 06, 2015
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Summary
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Here, we present a simple method for performing fluorescence DNA in situ hybridization (DNA ISH) to visualize repetitive heterochromatic sequences on slide-mounted chromosomes. The method requires minimal reagents and it is versatile for use with short or long probes, different tissues, and detection with fluorescence or non-fluorescence-based signals.
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Cite this Article
Larracuente, A. M., Ferree, P. M. Simple Method for Fluorescence DNA In Situ Hybridization to Squashed Chromosomes. J. Vis. Exp. (95), e52288, doi:10.3791/52288 (2015).
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