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Biology

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Simple Method for Fluorescence DNA In Situ Hybridization to Squashed Chromosomes
 

Simple Method for Fluorescence DNA In Situ Hybridization to Squashed Chromosomes

Article DOI: 10.3791/52288
January 6th, 2015

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Summary January 6th, 2015

Here, we present a simple method for performing fluorescence DNA in situ hybridization (DNA ISH) to visualize repetitive heterochromatic sequences on slide-mounted chromosomes. The method requires minimal reagents and it is versatile for use with short or long probes, different tissues, and detection with fluorescence or non-fluorescence-based signals.

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