Cd19 Cd27 Markers
Cd19 Cd27 Markers - Related Videos
03:01
Flow Cytometry-Based Cell Sorting for Different Circulatory B Cell Populations
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2025
In this video, we describe a protocol for the flow cytometry-based sorting of naïve B cell, memory B cell, and plasmablast plasma cell subpopulations from human peripheral blood B cells. The different B cell subpopulations can be distinguished based on fluorophore-based antibody binding to the CD19, CD27, and CD38 receptors expressed on the B cell surfaces, followed by cell sorting.
09:41
Imaging CD19+ B Cells in an Experimental Autoimmune Encephalomyelitis Mouse Model using Positron Emission Tomography
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Cited by 1 •
2023
This paper details methodology for radiolabeling a human-specific anti-CD19 monoclonal antibody and how to use it to quantify B cells in the central nervous system and peripheral tissues of a mouse model of multiple sclerosis using in vivo PET imaging, ex vivo gamma counting, and autoradiography approaches.
12:16
A Syngeneic Mouse B-Cell Lymphoma Model for Pre-Clinical Evaluation of CD19 CAR T Cells
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Cited by 17 •
2018
Here, we present a protocol for the production and pre-clinical testing of murine CD19 CAR T cells by retroviral transduction and utilization as a therapy against established syngeneic A20 B-cell lymphoma in BALB/c mice with or without lymphodepleting pre-conditioning.
09:38
Multiplexed Immunofluorescence Assay for Spatial Assessment of Senescence Markers in vivo
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2026
Multiplexed immunofluorescence enables sequential labeling of multiple antibody markers within a single tissue section to support spatial analysis of tissue architecture and cell populations. Here, a workflow for multiplex detection of senescence-associated, structural, and immune markers is presented, including manual and automated slide preparation, iterative imaging, and single-cell analysis using digital pathology software.
08:26
The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity
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Cited by 10 •
2016
Human peripheral blood is commonly used for the assessment of the humoral immune response. Here, the methods for isolating human B cells from peripheral blood, differentiating human B cells into antibody (Ab)-secreting B cells (ASCs) in culture, and enumerating the total IgM- and IgG-ASCs via an ELISpot assay are described.
FAQs
Using both markers creates a more informative phenotypic comparison than relying on either signal alone. CD19 helps establish the B-lineage population, while CD27 separates antigen-experienced, memory-like cells from many naïve B cells within that broader group. Their combined pattern therefore supports assessment of B-cell composition and memory-related changes in a sample.
CD27 provides a phenotypic indication of prior antigen experience because CD27-positive cells can represent memory-like B cells, whereas many naïve B cells do not show the same marker pattern. This distinction helps investigators examine whether a B-cell population contains evidence of memory formation, while recognizing that CD27 should be interpreted as part of a broader phenotype.
Coexpression is most useful when treated as one element of a broader phenotype. After CD19 identifies the B-lineage population and CD27 provides memory-related information, additional phenotypic features can refine how that population is characterized. This layered interpretation helps distinguish changes in B-cell composition from conclusions based on a single surface marker.
A basic analysis uses fluorescent antibodies against CD19 and CD27 during flow cytometry. The CD19 signal defines the B-lineage population, and CD27 staining then separates antigen-experienced, memory-like cells from many naïve B cells. Investigators can examine the resulting marker patterns and coexpression with other phenotypic features to compare B-cell populations.
These markers are useful when the question concerns how B-cell populations change during infection or after vaccination. Investigators can compare the representation of antigen-experienced, memory-like cells with the broader B-lineage population, then relate those patterns to memory formation and immune responses. The approach connects cellular phenotype with humoral immune changes in these settings.
In immunodeficiency, chronic immune activation, or differing disease states, CD19 and CD27 profiling can reveal shifts in B-cell composition. A change in the proportion or pattern of measured populations may provide evidence that B-cell development, memory-related compartments, or immune activation differs between conditions. Interpretation remains comparative and depends on broader phenotypic analysis.