Journal
/
Cancer Research
/
A Syngeneic Mouse B-Cell Lymphoma Model for Pre-Clinical Evaluation of CD19 CAR T Cells
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal Cancer Research
A Syngeneic Mouse B-Cell Lymphoma Model for Pre-Clinical Evaluation of CD19 CAR T Cells

A Syngeneic Mouse B-Cell Lymphoma Model for Pre-Clinical Evaluation of CD19 CAR T Cells

DOI:
10.3791/58492-v

•

12:16 min

•

October 16, 2018

•

, , , ,
1Manchester Cancer Research Centre Building, Department Cancer Sciences,University of Manchester, 2Celyad

Chapters

  • 00:04Title
  • 00:55Days 1 & 2: Preparation and Transfection of Plat-E Retroviral Packaging Cell Line
  • 02:11Day 3: Preparation of Virus-Containing Supernatant for Transduction and Isolation and In Vitro Activation of Mouse Splenic T Cells
  • 04:27Day 4: Transduction of Mouse T Cells
  • 06:43Day 5: Round 2 of Transduction
  • 08:33In Vitro Validation of CAR T Cell Activity
  • 09:53Results: Pre-Clinical Evaluation of CD19 CAR T Cells
  • 11:32Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol for the production and pre-clinical testing of murine CD19 CAR T cells by retroviral transduction and utilization as a therapy against established syngeneic A20 B-cell lymphoma in BALB/c mice with or without lymphodepleting pre-conditioning.

Tags

CD19 CAR T CellsSyngeneic Mouse ModelB-cell LymphomaPre-clinical EvaluationLymphodepletionAnti-cancer ActivityRetroviral TransductionEx Vivo Validation

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

A Syngeneic Mouse Model of Metastatic Renal Cell Carcinoma for Quantitative and Longitudinal Assessment of Preclinical Therapies

Tumor Engraftment in a Xenograft Mouse Model of Human Mantle Cell Lymphoma

A Syngeneic Pancreatic Cancer Mouse Model to Study the Effects of Irreversible Electroporation

Orthotopic Transplantation of Syngeneic Lung Adenocarcinoma Cells to Study PD-L1 Expression

A Spheroid Killing Assay by CAR T Cells

Using CRISPR/Cas9 to Knock Out GM-CSF in CAR-T Cells

Manufacturing Chimeric Antigen Receptor (CAR) T Cells for Adoptive Immunotherapy

Ultrasound-Guided Orthotopic Implantation of Murine Pancreatic Ductal Adenocarcinoma

A Syngeneic Orthotopic Osteosarcoma Sprague Dawley Rat Model with Amputation to Control Metastasis Rate

Natural Killer (NK) and CAR-NK Cell Expansion Method using Membrane Bound-IL-21-Modified B Cell Line

Read Article