Cho K1 Cells
Cho K1 Cells - Related Videos
08:15
Use of High-Throughput Automated Microbioreactor System for Production of Model IgG1 in CHO Cells
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Cited by 11 •
2018
A detailed protocol for the concurrent operation of 48 parallel cell cultures under varied conditions in a microbioreactor system is presented. Cell culture process, harvest and subsequent antibody titer analysis are described.
07:06
Primary Clarification of CHO Harvested Cell Culture Fluid using an Acoustic Separator
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Cited by 7 •
2020
Presented here is a protocol for the primary clarification of CHO cell culture using an acoustic separator. This protocol can be used for the primary clarification of shake flask cultures or bioreactor harvests and has the potential application for continuous clarification of the cell bleed material during perfusion bioreactor operations.
01:13
meta-Directing Deactivators: –NO2, –CN, –CHO, –CO2R, –COR, –CO2H
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2023
All meta-directing substituents are deactivating groups. These substituents withdraw electrons from the aromatic ring, making the ring less reactive toward electrophilic substitution. For example, the nitration of nitrobenzene is 100,000 times slower than that of benzene because of the deactivating effect of the nitro group. The first step in an electrophilic aromatic substitution is the addition of an electrophile to form a resonance-stabilized carbocation. The energy diagrams for the...
14:21
Dissection of Organizer and Animal Pole Explants from Xenopus laevis Embryos and Assembly of a Cell Adhesion Assay
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Cited by 2 •
2007
This video demonstrates the technique used for preparation of organizer and animal pole explants from Xenopus laevis embryos, including the use of the eyebrow knife - a specialized dissection tool made of one's eyebrow. The protocol for assembling an adhesion assay is also given, which probes for the presence of key adhesion molecules present on the surface organizer or animal pole cells that are critical for proper development.
09:57
A Versatile Method of Patterning Proteins and Cells
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Cited by 10 •
2017
This report describes a simple, easy to perform technique, using low pressure vacuum, to fill microfluidic channels with cells and substrates for biological research.
FAQs
A major experimental advantage comes from introducing a defined receptor or immune protein into the cells and then examining the resulting response. This design links a cellular outcome to a selected molecular component rather than to many variables present in primary tissue. In infection research, it can clarify whether receptor binding, microbial attachment, or toxin activity depends on that component.
These cells provide a controlled background in which researchers can examine selected host or pathogen-associated factors without the full complexity of primary tissues. Such reduction supports direct comparisons between experimental conditions and helps distinguish effects associated with a defined molecule or interaction. The resulting measurements can guide interpretation of host-pathogen mechanisms and experimental interventions.
Adherent growth supports maintenance as organized cell cultures for assay preparation and observation, while genetic flexibility allows expression of selected receptors, immune proteins, or pathogen-associated factors. Together, these properties make it possible to construct comparable experimental conditions and evaluate how changing a defined cellular component affects attachment, binding, toxin activity, or related responses.
A typical conceptual workflow begins by maintaining the cells as an adherent culture, followed by introducing the selected receptor or other factor through transfection. Researchers then use the modified culture in an assay designed around the interaction of interest, such as microbial attachment or receptor binding. Comparisons with differently configured cultures help associate outcomes with the introduced component.
The system can support studies of microbial attachment, receptor binding, toxin activity, and broader host-pathogen interactions. Its value is greatest when investigators need to examine one interaction under controlled conditions rather than model every feature of a primary tissue. Results can help compare molecular mechanisms and assess whether an experimental intervention changes a defined cellular outcome.
By expressing selected immune proteins or pathogen-associated factors, these cells can serve as a platform for building cell-based assays with reproducible experimental conditions. Researchers can use those assays to compare molecular mechanisms, evaluate interventions, and examine defined cellular responses. This controlled approach complements more complex biological systems by clarifying specific contributions within an immune or infection-related interaction.