Elisa System
Elisa System - Related Videos
10:06
The ELISA Method
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2023
An enzyme-linked immunosorbent assay (ELISA) is typically performed to detect the presence and/or amount of a target protein of interest within an experimental sample. Detection of the target protein is made possible by antibodies, which make the ELISA an immunoassay. Through a series of incubation and washing steps, these antibodies, which are frequently linked, or conjugated, to an enzyme, will detect protein coating the bottom of a well on a microtiter plate. When exposed to a substrate,...
14:22
ELISA Assays: Indirect, Sandwich, and Competitive
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2023
Source: Whitney Swanson1,2, Frances V. Sjaastad2,3, and Thomas S. Griffith1,2,3,4 1 Department of Urology, University of Minnesota, Minneapolis, MN 55455 2 Center for Immunology, University of Minnesota, Minneapolis, MN 55455 3 Microbiology, Immunology, and Cancer Biology Graduate Program, University of Minnesota, Minneapolis, MN 55455 4 Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455 Enzyme-linked immunosorbent assay (ELISA) is frequently used to measure the presence...
03:55
ELISA-Based Method to Analyze Interactions between Bait and Prey Proteins
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2025
This video describes an ELISA-based technique that detects protein-protein interactions between two proteins by measuring the binding of the second protein to the immobilized first protein. The bound second protein is detected using specific antibodies labeled with an enzyme, such as horseradish peroxidase.
03:35
ELISA-Based Reporter Assay to Study the Receptor-Ligand Interaction in BW Cells
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2025
This video demonstrates the reporter assay-based detection of the receptor-ligand interaction. The successful receptor-ligand interaction activates the downstream signaling pathway, causing the generation and release of cytokines as secretory reporter molecules in the solution, which is confirmed using ELISA.
12:38
Titration ELISA as a Method to Determine the Dissociation Constant of Receptor Ligand Interaction
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Cited by 29 •
2018
A detailed protocol to perform a titration ELISA is described. Moreover, a novel algorithm is presented to evaluate titration ELISAs and to obtain a dissociation constant of binding of a soluble ligand to a microtiter plate-immobilized receptor.
FAQs
Specific immune recognition provides the main selectivity. The target binds either to a surface or to a capture antibody, and an enzyme-linked detection antibody recognizes the resulting immune complex. Because the measured signal depends on these coordinated binding events, the assay can focus on a particular antigen or antibody within a complex biological sample.
The assay’s target changes, but the central principle remains specific immune binding followed by enzyme-based detection. Antigen-focused formats support detection of pathogen-associated molecules, whereas antibody-focused formats can reveal an immune response to a target. This distinction makes the system useful for both pathogen investigation and serological assessment.
The enzyme converts the final substrate into a measurable color signal, linking an invisible binding event to an observable result. Within the assay’s working range, greater target abundance produces a stronger color change. This conversion allows researchers to quantify immune mediators, antigens, or antibodies rather than only recording whether binding occurred.
Quantitation comes from relating color intensity to the amount of target present in the biological sample. The assay therefore provides more than a positive or negative classification: it can estimate levels of specific antigens, antibodies, or cytokines. That capability is especially relevant when comparing immune responses, infection-related measurements, or vaccine-associated outcomes.
A typical workflow establishes target binding at a plate surface, uses a capture antibody when appropriate, and then introduces an enzyme-linked detection antibody to form a specific immune complex. Adding substrate generates the readout, and the resulting color is measured to estimate target abundance. The plate-based format supports consistent processing across multiple samples.
Researchers can apply the system to serological testing, pathogen detection, vaccine evaluation, and measurement of immune mediators such as cytokines. Its sensitivity, adaptability, and compatibility with standardized plate-based workflows make it useful when studies require comparable measurements across biological samples, including investigations of immune responses during infection or after vaccination.