Fluorescence Measurement Technique
Fluorescence Measurement Technique - Related Videos
03:04
Measurement of Extracellular Fluorescent Glucose Analog Depletion: A Surrogate Measurement Technique to Assess Glucose Uptake in Mouse Organs Ex Vivo
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2025
In this video, we demonstrate an ex vivo technique to measure extracellular glucose analog depletion in harvested mouse organs. It involves incubating the mouse tissue in a solution containing fluorescently-labeled deoxyglucose, which is taken up by the cells. The glucose analog uptake can be indirectly determined by measuring the fluorescence of the incubated medium.
14:39
Measuring Phagosome pH by Ratiometric Fluorescence Microscopy
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Cited by 26 •
2015
Phagosomal pH influences phagosome maturation, oxidant production, phagosomal killing as well as antigen presentation. Here we describe a ratiometric method for measuring time-course and endpoint pH changes in individual phagosomes in living phagocytes using fluorescence microscopy.
08:28
Measurement of Force-Sensitive Protein Dynamics in Living Cells Using a Combination of Fluorescent Techniques
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Cited by 3 •
2018
Here, we present a protocol for the simultaneous use of Förster resonance energy transfer-based tension sensors to measure protein load and fluorescence recovery after photobleaching to measure protein dynamics enabling the measurement of force-sensitive protein dynamics within living cells.
07:03
In Situ Measurement of Vacuum Window Birefringence using 25Mg+ Fluorescence
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2020
Presented here is a method to measure the birefringence of vacuum windows by maximizing the fluorescence counts emitted by Doppler cooled 25Mg+ ions in an ion trap. The birefringence of vacuum windows will change the polarization states of the laser, which can be compensated by changing the azimuthal angles of external wave plates.
09:31
Noninvasive Blood Pressure Measurement Techniques
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2023
Source: Hamna J. Qureshi and Craig J. Goergen, Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana Here we will highlight the key similarities and differences of noninvasive blood pressure measurement techniques between humans and rodents and examine the engineering principles that govern blood pressure. The principles that govern current cuff technology to acquire systolic and diastolic pressures will also be discussed. Commercially available cuffs that connect...
FAQs
The signal is shaped by the fluorophore’s energy transition. After absorbing shorter-wavelength light, the molecule enters an excited electronic state and releases part of that energy as longer-wavelength emission. Measuring this shifted light separates the emitted signal from the excitation source and provides the basis for fluorescence intensity or spectral analysis.
Intensity and spectrum answer different measurement needs. Intensity indicates how much fluorescence is detected, whereas a spectrum records the distribution of emitted light across wavelengths. In immunology and infection studies, intensity can support relative-abundance comparisons, while spectral information characterizes the emitted signal. Interpreting either output requires relating it to the fluorescent label and biological target.
The label determines which biological feature becomes measurable. Antibodies can direct fluorescence toward particular molecules, nucleic-acid probes toward corresponding nucleic-acid targets, and cellular markers toward defined cell features. Because these labels can be associated with pathogens, immune cells, or specific molecules, the resulting signal can provide information about presence, location, or relative abundance in a sample.
An experiment first links a fluorescent label to an antibody, nucleic-acid probe, or cellular marker associated with the biological feature of interest. The labeled material is then examined with an appropriate platform, such as an immunoassay, microscope, flow cytometer, or diagnostic analysis system. The instrument records intensity or spectral output for interpretation.
Fluorescence measurement supports several complementary research formats. In immunoassays, microscopy, flow cytometry, and diagnostic analysis, fluorescent labels help researchers examine biological targets using measured signal. The same underlying readout can contribute to detection, localization, or relative-abundance assessment, depending on the labeled target and analytical format. This flexibility supports experimental studies and diagnostic analysis.
In immunology and infection research, the technique connects a detected fluorescent signal to questions about host-pathogen interactions and immune responses. Labels can make pathogens, immune cells, or specific molecules visible or quantifiable within an analysis. This supports examination of whether a target is present, where it is located, and its relative abundance in biological samples.