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JoVE Journal
Immunology and Infection

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Measuring Phagosome pH by Ratiometric Fluorescence Microscopy
 

Measuring Phagosome pH by Ratiometric Fluorescence Microscopy

Article DOI: 10.3791/53402
December 7th, 2015

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Summary December 7th, 2015

Phagosomal pH influences phagosome maturation, oxidant production, phagosomal killing as well as antigen presentation. Here we describe a ratiometric method for measuring time-course and endpoint pH changes in individual phagosomes in living phagocytes using fluorescence microscopy.

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