Hiv-specific Cd4 T Cells
Hiv-specific Cd4 T Cells - Related Videos
08:11
Assessing the Innate Sensing of HIV-1 Infected CD4+ T Cells by Plasmacytoid Dendritic Cells Using an Ex vivo Co-culture System.
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Cited by 1 •
2015
Unlike cell-free HIV-1 particles, infected CD4+ T cells are effectively sensed by plasmacytoid dendritic cells (pDCs). This manuscript describes a method where peripheral blood mononuclear cells (PBMCs) or isolated pDCs are co-cultured with HIV-1 infected T cells to evaluate innate sensing by pDCs as assessed by release of type-I IFN.
12:44
CD4+ T-Lymphocyte Capture Using a Disposable Microfluidic Chip for HIV
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Cited by 5 •
2007
12:07
Preparation and Use of HIV-1 Infected Primary CD4+ T-Cells as Target Cells in Natural Killer Cell Cytotoxic Assays
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Cited by 12 •
2011
Cytotoxicity assays to measure natural killer cell lytic responses to HIV-infected cells is limited by the purity of the target cells. We demonstrate here the isolation of a highly purified population of HIV-1 infected primary T-cell blasts by taking advantage of HIV-1 s ability to down-modulate CD4.
09:26
In Vitro Assay to Evaluate the Impact of Immunoregulatory Pathways on HIV-specific CD4 T Cell Effector Function
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Cited by 5 •
2013
We developed an in vitro assay to investigate the role of immunoregulatory pathways in the regulation of cytokine secretion by HIV-specific CD4 T cells.
13:47
Development of Cell-type specific anti-HIV gp120 aptamers for siRNA delivery
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Cited by 36 •
2011
Several 2’-Fluoro RNA aptamers against HIV-1Ba-L gp120 with nanomole affinity are isolated from a RNA library by in vitro SELEX procedure. A new dual inhibitory function anti-gp120 aptamer-siRNA chimera is created and shows considerable promise for systemic anti-HIV therapy.
FAQs
Antigen-presenting cells process HIV-derived peptides and display them on major histocompatibility complex class II molecules. Recognition by the T-cell receptor supplies the antigen-specific trigger for activation. The resulting response can include cytokine production and coordinated help for B cells and HIV-specific CD8 T cells, linking recognition to broader antiviral immunity.
These cells have a dual biological role in HIV infection. Their activation can coordinate immune responses, yet expression of the CD4 receptor and chemokine coreceptors allows HIV to enter them. Consequently, an antigen-responsive population may also undergo viral replication, depletion, or latent infection. This tension complicates interpretation of their contribution to durable viral control.
A useful assessment goes beyond counting cells. Frequency indicates how prevalent the HIV-responsive population is, while function captures activities such as cytokine production and support for B cells or CD8 T cells. Phenotype adds information about cellular state. Together, these dimensions distinguish the presence of responsive cells from their potential contribution to immune control.
Studies can characterize HIV-specific CD4 T-cell populations through three complementary dimensions: frequency, function, and phenotype. Frequency addresses abundance, function addresses activities such as cytokine production and immune support, and phenotype contributes distinguishing cellular information. Considering these dimensions together provides a more informative immune profile than any single measure, supporting interpretation of antiviral responses.
Vaccine evaluation can use these cells as an immune-monitoring readout because studies can examine their frequency, function, and phenotype. A response may be considered in terms of whether HIV recognition is accompanied by cytokine production and help for B cells or HIV-specific CD8 T cells. This links vaccine-related immune responses to coordinated antiviral activity.
Examining these cells helps researchers relate immune responsiveness to HIV persistence. The same population that recognizes HIV may be exposed to infection through CD4 and chemokine coreceptors, creating possible links to viral replication, depletion, or latent infection. Tracking frequency, function, and phenotype therefore informs research on persistence and strategies for durable HIV control.