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Immunology and Infection

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JoVE Journal Immunology and Infection
In Vitro Assay to Evaluate the Impact of Immunoregulatory Pathways on HIV-specific CD4 T Cell Effector Function
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In Vitro Assay to Evaluate the Impact of Immunoregulatory Pathways on HIV-specific CD4 T Cell Effector Function

Article DOI: 10.3791/50821 • 09:26 min • October 15th, 2013
October 15th, 2013 •
*1, *1, 1, 1,2, 1,2
1The Ragon Institute of MGH, MIT and Harvard, 2Centre de Recherche du Centre Hospitalier de l'Université de Montréal (CRCHUM)
* These authors contributed equally

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Summary
Automatic Translation

We developed an in vitro assay to investigate the role of immunoregulatory pathways in the regulation of cytokine secretion by HIV-specific CD4 T cells.

Tags

In Vitro Assay Immunoregulatory Pathways HIV-specific CD4 T Cell Effector Function T Cell Exhaustion Chronic Viral Infections PD-1 IL-10 Antigen Exposure Proliferation Cytolytic Function Cytokine Production Blocking Antibodies LCMV Infection Cytokine Secretion Human Samples Experimental Design CD8 T Cells PBMCs Ficoll Separation Method PD-L1 IL-10Rα HIV-1 Gag Peptide Pool Supernatant Analysis Phenotypic Analysis
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