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Immunology and Infection

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Development of Cell-type specific anti-HIV gp120 aptamers for siRNA delivery

Article DOI: 10.3791/2954 • 13:48 min • June 23rd, 2011
June 23rd, 2011 •
1, 1, 2, 3, 1
1Department of Molecular and Cellular Biology, Beckman Research Institute of City of Hope, 2Graduate School of Biological Sciences, Beckman Research Institute of City of Hope, 3Shared Resource-DNA/RNA Peptide, Beckman Research Institute of City of Hope

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Summary
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Several 2’-Fluoro RNA aptamers against HIV-1Ba-L gp120 with nanomole affinity are isolated from a RNA library by in vitro SELEX procedure. A new dual inhibitory function anti-gp120 aptamer-siRNA chimera is created and shows considerable promise for systemic anti-HIV therapy.

Tags

Cell-type Specific Anti-HIV Gp120 Aptamers SiRNA Delivery HIV Infection Antiretroviral Agents Small Interfering (si)RNAs Therapeutics RNAi-based HIV/AIDS RNA Transcripts Efficient Delivery Targeted Intracellular SiRNA Delivery Approach Specific Cell Populations Or Tissues HIV-1 Gp120 Protein Glycoprotein Envelope Viral Entry CD4 Cells Anti-viral Strategy Drug Discovery 2'-F Modified Anti-HIV Gp120 RNA Aptamers Nitrocellulose Filter SELEX Method
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