Hydrodynamic Gene Transfer
Hydrodynamic Gene Transfer - Related Videos
12:54
Transient Expression of Proteins by Hydrodynamic Gene Delivery in Mice
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Cited by 26 •
2014
In vivo transfection of naked DNA by hydrodynamic gene delivery introduces genes into the tissue of an animal with minimal inflammatory response. Sufficient amounts of gene product are generated such that gene function and regulation as well as protein structure and function can be analyzed.
03:48
Hydrodynamic Gene Delivery: A Technique for In Vivo Transfection of Exogenous DNA in Murine Hepatocytes via Tail Vein Injection
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2025
This video describes the technique of genetic in vivo transfection of murine hepatocytes by hydrodynamic tail vein injection. This transfection system generates a long-term expression of the transgene in the hepatocytes.
01:27
Horizontal Gene Transfer
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2025
Horizontal gene transfer (HGT) is a process where genetic material moves between organisms within the same generation, unlike vertical gene transfer, which occurs from parent to offspring. HGT plays a crucial role in microbial evolution, adaptation, and survival, particularly in shared environments like the human gut.Mobile genetic elements such as plasmids, prophages, integrons, insertion sequences, and transposons facilitate this process. HGT occurs through three primary mechanisms:...
10:13
A Microfluidic-based Hydrodynamic Trap for Single Particles
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Cited by 14 •
2011
In this article, we present a microfluidic-based method for particle confinement based on hydrodynamic flow. We demonstrate stable particle trapping at a fluid stagnation point using a feedback control mechanism, thereby enabling confinement and micromanipulation of arbitrary particles in an integrated microdevice.
03:36
Production of Retroviral Particles for Gene Transfer
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2026
Source: Zjablovskaja, P., et al., Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells. J. Vis. Exp. (2018)This video demonstrates the production of recombinant retroviral particles by transfecting human kidney-derived packaging cells, enabling stable gene delivery to target cells for downstream applications such as genetic modification, functional assays, or lineage differentiation studies.
FAQs
A rapid, high-volume injection produces a sudden rise in vascular pressure, which distends blood vessels and transiently changes cell membranes. These physical effects increase the opportunity for circulating plasmid DNA to cross into tissues. After tail-vein delivery in mice, hepatocytes are particularly accessible, making the liver a central target for short-term in vivo gene expression.
Temporary expression allows investigators to control when an immune-related gene becomes active and how much genetic material is delivered, without establishing a stable transgenic line. This is valuable when cytokines, antigens, or receptors need to be examined during a defined experimental interval, helping connect gene activity with a time-limited host response.
Delivery route and the physical conditions of injection strongly influence tissue exposure. In the described mouse model, tail-vein administration directs circulating plasmid DNA toward the liver, where hepatocytes show prominent uptake. The injected nucleic acid type also matters, because plasmid DNA provides a vehicle for temporary production of the selected immune or infection-related gene.
Hydrodynamic gene transfer produces temporary gene expression rather than permanently incorporating a new genetic program into an inherited animal line. That distinction reduces the need to develop and maintain stable transgenic models when the research question concerns a defined exposure period. It also supports experimental designs centered on controlled timing and dosage of gene activity.
A typical workflow selects a plasmid carrying the gene of interest, administers it through a rapid, high-volume injection, and then examines the resulting expression or biological response in vivo. In mice, tail-vein delivery is used when liver-directed exposure is desired. Investigators can then relate cytokine, antigen, receptor, or other gene activity to the study outcome.
The approach is useful when researchers need controlled in vivo expression of genes involved in host defense or infection biology. It can support host-response studies, genetic vaccine evaluation, disease-pathway modeling, and testing of therapeutic strategies. Because expression is temporary and the delivered amount can be controlled, experiments can align gene activity with a selected phase of the investigation.