Cem-based T Cell Line
Cem-based T Cell Line - Related Videos
05:14
Studying Interactions between Myeloid Cells and CAR T Cells In Vitro and In Vivo
0 Views •
2025
Given the limited xenograft models available to study interactions between human CART and myeloid cells, we established in vitro and in vivo models to understand the impacts of human macrophages on CART cells. Findings can potentially be generalized to evaluate macrophage roles in the tumor microenvironment and test macrophage-targeted immunotherapies.
10:33
Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells
0 Views •
Cited by 34 •
2019
Here, we present several simple methods for evaluating viability and death in 3D cancer cell spheroids, which mimic the physico-chemical gradients of in vivo tumors much better than the 2D culture. The spheroid model, therefore, allows evaluation of the cancer drug efficacy with improved translation to in vivo conditions.
10:12
Measuring Endoplasmic Reticulum Stress and Unfolded Protein Response in HIV-1 Infected T-Cells and Analyzing its Role in HIV-1 Replication
0 Views •
Cited by 1 •
2024
Here, we describe some established methods to determine endoplasmic reticulum (ER) stress and unfolded protein response (UPR) activation, with particular emphasis on HIV-1 infection. This article also describes a set of protocols to investigate the effect of ER stress/UPR on HIV-1 replication and virion infectivity.
10:28
Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
0 Views •
Cited by 7 •
2018
Regulation of the chromatin environment is an essential process required for proper gene expression. Here, we describe a method for controlling gene expression through the recruitment of chromatin-modifying machinery in a gene-specific and reversible manner.
08:04
In Vitro Tumor Cell Rechallenge For Predictive Evaluation of Chimeric Antigen Receptor T Cell Antitumor Function
0 Views •
Cited by 23 •
2019
Here, we describe an in vitro co-culture method to recursively challenge tumor-targeted T cells, which allows for phenotypic and functional analysis of antitumor T cell activity.
FAQs
Surface receptors and co-receptors provide cellular binding sites that can interact with pathogen envelope components. These interactions help investigators examine whether a pathogen can attach to, enter, or preferentially infect a particular T-lineage model. By observing changes in infection-related outcomes, researchers can investigate tropism and receptor-dependent mechanisms under controlled laboratory conditions.
Suspension growth allows the cells to remain dispersed in the culture environment rather than forming an adherent layer. This supports access to a relatively consistent population of T-lineage cells during controlled assays. The resulting experimental accessibility and reproducibility help researchers compare pathogen exposure, immune signaling, cytotoxicity, or antiviral responses across conditions.
These cell lines provide a reproducible laboratory system in which specific infection or immune-response questions can be examined with fewer sources of biological variation than may occur in primary cells. They do not replace primary-cell or animal models, but they can complement them by enabling controlled analyses of pathogen replication, cytotoxicity, signaling, and antiviral activity.
A study generally maintains the cells in suspension culture, exposes them to the pathogen or experimental treatment, and then evaluates outcomes relevant to the question. Measurements may focus on pathogen replication, cellular cytotoxicity, antiviral activity, or immune-cell responses. Because the system is accessible and reproducible, investigators can compare these outcomes across controlled experimental conditions.
CEM-based systems are particularly useful for asking how pathogens interact with T-lineage cells, whether cellular receptors support entry, and how infection affects cell survival or immune signaling. They also support testing of antiviral activity and examining cytotoxic effects. These applications make the model relevant to both infectious-disease experiments and broader studies of T-cell biology.
Assays can provide information about pathogen tropism, replication, cytotoxicity, antiviral effects, and immune-cell responses. For example, a controlled comparison may reveal whether a pathogen preferentially interacts with the model, whether treatment suppresses replication, or whether exposure produces harmful cellular effects. Interpreting several outcomes together helps connect receptor-mediated interactions with broader infection and immune consequences.