Ige Expressing B Cells
Ige Expressing B Cells - Related Videos
09:40
Detection of True IgE-expressing Mouse B Lineage Cells
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Cited by 3 •
2014
In vitro analysis of class switch recombination in mice is challenging due to cytophilic IgE molecules bound to Fc receptors on the surface of B cells. We describe a method for IgE detection using trypsin-mediated cleavage of surface-bound IgE prior to fixation and permeabilization for cytoplasmic fluorescence staining.
10:22
Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity
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Cited by 14 •
2011
Basophil activation test is a potent tool for the detection of IgE-dependent allergies in vitro. Here, an optimized protocol for basophil activation test is used to investigate drug hypersensitivity. A method for the efficient production of covalent drug-protein conjugates and their physicochemical characterization is described.
01:25
Antiasthma Drugs: Mast Cell Stabilizers and Anti-IgE Drugs
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2024
Asthma is a chronic respiratory condition for which new therapeutic avenues, including anti-inflammatory drugs like mast cell stabilizers and anti-IgE treatments, continue to be developed. Mast cell stabilizers, such as cromolyn (also known as sodium cromoglycate) and nedocromil (Tilade), are effective drugs in asthma management. These stabilizers hinder histamine release by skillfully obstructing the activation of mast cells and other cellular entities. Notably, they navigate this task without...
10:34
Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
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Cited by 2 •
2010
This procedure describes a quick and easy workflow to introduce siRNA into difficult to transfect cell lines and follow gene expression by real-time PCR. Use of an automated cell counter, multi-well electroporation plate, and automated electrophoresis station provide quick and reliable results without the need for expensive robotic handling.
01:58
Cell Specific Gene Expression
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2020
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
FAQs
Activated B cells are directed toward the ε heavy-chain gene when they receive interleukin-4 or interleukin-13 together with CD40-mediated T-cell help. These signals promote class-switch recombination, changing the immunoglobulin heavy-chain program toward IgE expression. This coordinated input connects antigen-driven B-cell activation with the antibody pathway associated with allergic inflammation and parasite defense.
CD40-mediated T-cell help matters because it acts alongside interleukin-4 or interleukin-13 during the switch to the ε heavy-chain gene. The paired signals illustrate that IgE expression depends on coordinated communication between T cells and B cells. This interaction provides a mechanistic link between immune-cell cooperation and the generation of IgE-producing or IgE-displaying B-cell populations.
Some switched B cells can differentiate into IgE-secreting plasma cells, creating a source of secreted antibody. Other IgE-expressing B cells are considered in relation to antibody display on the B-cell surface. Tracking these outcomes connects B-cell differentiation with downstream receptor activation and with the distinct cellular stages examined in allergy and infection research.
Once IgE is bound to high-affinity receptors on mast cells and basophils, those cells are positioned to respond rapidly when the antigen is recognized. The result is mediator release, linking an earlier B-cell antibody response to immediate cellular activity. This receptor-based step helps explain why IgE-expressing B cells are central to rapid allergic inflammation.
The same IgE-centered biology has two major contexts. In allergy, receptor-bearing mast cells and basophils support rapid mediator release after antigen recognition. In defense against certain parasites, IgE represents an antibody response associated with infection protection. Studying the B-cell stage therefore connects allergic inflammation with host defense rather than treating them as unrelated phenomena.
Research on these B cells can identify points where the IgE response is initiated, shaped, or connected to effector-cell activation. That information supports the development of targeted therapies by linking upstream B-cell signals, antibody production, and downstream mast-cell or basophil responses. The value is mechanistic: therapy design can be informed by the pathway being studied.