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JoVE Journal
Biology

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Using an Automated Cell Counter to Simplify Gene Expression Studies
 

Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells

Article doi: 10.3791/1904
April 14th, 2010

Summary April 14th, 2010

This procedure describes a quick and easy workflow to introduce siRNA into difficult to transfect cell lines and follow gene expression by real-time PCR. Use of an automated cell counter, multi-well electroporation plate, and automated electrophoresis station provide quick and reliable results without the need for expensive robotic handling.

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