In Vitro Infection
In Vitro Infection - Related Videos
07:57
An In Vitro Bladder Model of Catheter-Associated Urinary Tract Infection
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Cited by 1 •
2025
This protocol details an in vitro model of the catheterized urinary tract, which can be used to study both planktonic and biofilm-associated bacterial cell populations in simulated catheter-associated urinary tract infections. This model can be further utilized to study the efficacy of antimicrobial products aimed at controlling urinary tract infections.
06:36
PRP as a New Approach to Prevent Infection: Preparation and In vitro Antimicrobial Properties of PRP
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Cited by 35 •
2013
Implant-associated infection is a significant clinical complication. This study describes an approach using platelet-rich plasma (PRP) to prevent implant-associated infections, presents the protocol for preparing PRP with constant platelet concentration, and reports the newly identified antimicrobial properties of PRP and related protocols for examining such antimicrobial properties in vitro.
03:56
An In Vitro Recombinant Virus Reporter System for the Detection of Viral Infection
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2025
This video demonstrates an in vitro technique to detect hepatitis B virus (HBV) infection via a recombinant HBV reporter system. Upon infecting host cells, the recombinant viral genome expresses the luciferase reporter enzyme, which produces bioluminescence upon oxidizing its substrate — helping detect viral infection.
11:14
Detection of Low Copy Number Integrated Viral DNA Formed by In Vitro Hepatitis B Infection
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Cited by 3 •
2018
We describe here the in vitro generation of HBV DNA via a Hepatitis B virus infection system and the highly sensitive detection of its (1–2 copies) integration using inverse nested PCR.
02:49
Generation of an In Vitro Cell Culture Model of Malaria-HIV Co-Infection
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2025
This video demonstrates the generation of an in vitro malaria-HIV co-infection cell culture model. HIV-infected peripheral blood mononuclear cells co-cultured with Plasmodium falciparum parasitized erythrocytes potentially support each other's growth and provide insights into the immune responses and disease progression in HIV and malaria co-infections.
FAQs
The model allows researchers to control the pathogen dose and the cellular or tissue environment while observing infection-related events. This control helps separate specific host-pathogen interactions from other variables that are difficult to isolate in vivo. As a result, investigators can examine defined stages of infection and compare responses under consistent laboratory conditions.
Researchers can follow a sequence that includes pathogen attachment, cellular entry, replication, and release. Monitoring these stages helps determine where host or pathogen factors influence infection. The same model can also show when cultured cells activate innate or adaptive immune responses, linking changes in pathogen behavior with corresponding host responses.
In vitro infection provides a tractable setting for examining selected parts of infection without the full complexity of a living organism. Researchers can focus on specific cells, tissues, or engineered culture systems and control experimental variables more directly. This makes the approach useful for clarifying mechanisms that may be difficult to isolate in vivo.
After pathogen exposure, researchers monitor activation of innate or adaptive immune responses in the cultured system. These observations can reveal how host cells respond to particular infection stages and help identify immune mechanisms involved in pathogen control or interaction. The approach therefore connects cellular infection events with immunological outcomes under defined conditions.
A typical workflow begins with an isolated cell, tissue, or engineered culture system, followed by introduction of a defined pathogen dose. Researchers then monitor attachment, entry, replication, release, and immune activation. The selected observations depend on the research question, such as comparing pathogen behavior, examining host responses, or evaluating a treatment.
Using the same controlled culture context, researchers can expose comparable host systems to different pathogen strains and examine their interactions with the cells or tissues. Differences in attachment, entry, replication, release, or immune activation can then be assessed across strains. This supports characterization of strain-specific behavior without changing the broader experimental conditions.
Researchers can introduce a treatment into a controlled infection model and examine its effects on pathogen-related processes or host responses. Measurements of replication, release, and immune activation help determine how the intervention changes the infection system. These results provide experimental evidence for comparing treatments and selecting approaches for further study.
These models support disease modeling, investigation of host-pathogen interactions, identification of immune mechanisms, and evaluation of potential vaccines or therapeutics. Because researchers can control the pathogen exposure and culture context, the systems are useful for generating focused evidence before findings are used to guide broader disease or treatment research.