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Immunology and Infection
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PRP as a New Approach to Prevent Infection: Preparation and In vitro Antimicrobial Properties of PRP
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Immunology and Infection
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JoVE Journal Immunology and Infection
PRP as a New Approach to Prevent Infection: Preparation and In vitro Antimicrobial Properties of PRP

PRP as a New Approach to Prevent Infection: Preparation and In vitro Antimicrobial Properties of PRP

DOI:
10.3791/50351-v

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06:36 min

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April 09, 2013

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1Department of Orthopaedics, School of Medicine,West Virginia University , 2Department of Orthopaedics, Stem Cell Research Center,University of Pittsburgh, 3WVNano Initiative, 4Mary Babb Randolph Cancer Center

Chapters

  • 00:05Title
  • 01:36Preparation of PRP
  • 03:01In vitro Antimicrobial Test of PRP: The Kill Curve Assay
  • 04:54Results: PRP Approach to Prevent Infection
  • 05:53Conclusion

Summary

Automatic Translation

Automatic Translation

Implant-associated infection is a significant clinical complication. This study describes an approach using platelet-rich plasma (PRP) to prevent implant-associated infections, presents the protocol for preparing PRP with constant platelet concentration, and reports the newly identified antimicrobial properties of PRP and related protocols for examining such antimicrobial properties in vitro.

Tags

Keywords: Platelet-rich Plasma (PRP)Antimicrobial PropertiesImplant-associated InfectionAntibiotic ResistanceOrthopaedic InfectionsMethicillin-sensitive Staphylococcus AureusMethicillin-resistant Staphylococcus AureusGroup A StreptococcusNeisseria GonorrhoeaeInfection PreventionIn Vitro Antimicrobial ActivityBiocompatibleTwice Centrifugation Approach

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