Intracerebral Injection
Intracerebral Injection - Related Videos
10:44
Modeling Intracerebral Hemorrhage in Mice: Injection of Autologous Blood or Bacterial Collagenase
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Cited by 60 •
2012
Clinically relevant animal models of intracerebral hemorrhage (ICH) are needed to extend our knowledge of hemorrhagic stroke and to examine novel therapeutic strategies. In this study, we describe and evaluate two ICH models that implement unilateral injections of either autologous whole blood or bacterial collagenase into the basal ganglia (corpus striatum) of mice.
07:02
Autologous Blood Injection to Model Spontaneous Intracerebral Hemorrhage in Mice
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Cited by 28 •
2011
The autologous blood injection model of intracerebral hemorrhage in mice described in this protocol uses the double injection technique to minimize risk of blood reflux up the needle track, no anticoagulants in the pumping system, and eliminates all dead space and expandable tubing in the system.
09:41
Intrastriatal Injection of Autologous Blood or Clostridial Collagenase as Murine Models of Intracerebral Hemorrhage
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Cited by 35 •
2014
Preclinical models of intracerebral hemorrhage are utilized to mimic certain aspects of clinical disease. Thus, mechanisms of injury and potential therapeutic strategies may be explored. In this protocol, two models of intracerebral hemorrhage are described, intrastriatal (basal ganglia) injections of autologous blood or collagenase.
09:07
In Vivo Intracerebral Stereotaxic Injections for Optogenetic Stimulation of Long-Range Inputs in Mouse Brain Slices
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Cited by 7 •
2019
This protocol describes a set of methods to identify the cell-type specific functional connectivity of long-range inputs from distant brain regions using optogenetic stimulations in ex vivo brain slices.
06:58
Non-restraining EEG Radiotelemetry: Epidural and Deep Intracerebral Stereotaxic EEG Electrode Placement
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Cited by 21 •
2016
Non-restraining EEG radiotelemetry is a valuable methodological approach to record in vivo long-term electroencephalograms from freely moving rodents. This detailed protocol describes stereotaxic epidural and deep intracerebral electrode placement in different brain regions in order to obtain reliable recordings of CNS rhythmicity and CNS related behavioral stages.
FAQs
Stereotactic coordinates identify a selected brain region so the injected material reaches a defined anatomical target. This spatial control helps researchers attribute observed effects to the intended tissue rather than to imprecise deposition elsewhere. In infection and immunology studies, accurate targeting is especially important when comparing pathogen spread, local tissue injury, or immune activity between experimental groups.
Direct delivery removes the blood-brain barrier as a limiting factor for the administered substance, allowing researchers to examine its effects within brain tissue under controlled local exposure. This design can distinguish responses caused by activity at the injection site from outcomes that depend on passage through the circulation, which is relevant when evaluating neuroinflammation, infection, or treatment responses.
The selected brain region, anatomical coordinates, deposited volume, and placement accuracy all influence the resulting response. These variables determine where exposure occurs and how much material reaches the target while affecting the likelihood of off-target effects. Keeping them controlled supports more reliable comparisons of pathogen behavior, tissue damage, immune-cell activity, and therapeutic efficacy.
Intracerebral injection establishes a localized exposure within brain tissue, whereas delivery through the circulation must contend with the blood-brain barrier before reaching the central nervous system. This distinction makes the technique useful for studying regional effects directly. It also means that findings primarily describe responses under controlled local exposure rather than unrestricted whole-body distribution.
The procedure uses stereotactic guidance to align the experiment with defined brain coordinates. A fine needle is then positioned at the selected site, and a controlled volume of cells, pathogens, drugs, or another test substance is deposited. These elements provide a reproducible workflow for limiting off-target exposure and relating later observations to a specific brain region.
Researchers apply it when they need controlled models of central nervous system infection, neuroinflammation, or host immune responses. Introducing a defined substance into a selected region enables investigation of pathogen spread and tissue injury, while also supporting analysis of immune-cell activity. The same framework can be used to assess how candidate treatments affect these localized processes.
These models can provide information about how pathogens spread through brain tissue, how much injury develops, and how immune cells respond at or near the exposure site. They can also support comparisons of treatment efficacy under controlled conditions. Interpreting these outcomes requires considering both the targeted region and the localized nature of the experimental exposure.