Interferon Gamma Elisa
Interferon Gamma Elisa - Related Videos
05:28
Assay for the Identification of Interferon-Gamma Secretion in Murine Lymphoid Organs
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2025
This video demonstrates an imaging method to visualize in situ interferon-gamma (IFN-γ) secretion in the mouse spleen. Following bacterial infection and the induction of IFN-γ accumulation inside T cells and natural killer cells, the spleen is harvested from the mouse. Upon obtaining tissue sections, immunostaining is performed to visualize IFN-γ inside the cells.
10:06
The ELISA Method
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2023
An enzyme-linked immunosorbent assay (ELISA) is typically performed to detect the presence and/or amount of a target protein of interest within an experimental sample. Detection of the target protein is made possible by antibodies, which make the ELISA an immunoassay. Through a series of incubation and washing steps, these antibodies, which are frequently linked, or conjugated, to an enzyme, will detect protein coating the bottom of a well on a microtiter plate. When exposed to a substrate,...
09:11
Imaging of In Situ Interferon Gamma Production in the Mouse Spleen following Listeria monocytogenes Infection
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Cited by 3 •
2019
Here, we describe a simple confocal imaging method to visualize the in situ localization of cells secreting the cytokine Interferon gamma in murine secondary lymphoid organs. This protocol can be extended for the visualization of other cytokines in diverse tissues.
08:13
Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination
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Cited by 3 •
2019
The development of the interferon-gamma ELISpot assay for guinea pig PBMCs allows the characterization of T-cell responses in this highly relevant model for studying infectious diseases. We have applied the assay to measure T cell responses associated with intradermal delivery of DNA vaccines.
10:10
Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses
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2016
This protocol describes how to inoculate C57BL/6J mice with the EGD strain of Listeria monocytogenes (L. monocytogenes) and to measure interferon-γ (IFN-γ) responses by natural killer (NK) cells, natural killer T (NKT) cells, and adaptive T lymphocytes post-infection. This protocol also describes how to conduct survival studies in mice after infection with a modified LD50 dose of the pathogen.
FAQs
The paired-antibody arrangement provides the assay’s analytical basis. A capture antibody retains IFN-γ from the biological sample, while a second antibody linked to an enzyme binds the captured cytokine, creating a detectable complex. This two-antibody recognition connects the target cytokine to the final signal and supports quantitative measurement in immune-response studies.
After the antibody-antigen complex forms, the added substrate undergoes an enzyme-dependent color reaction. Because the resulting color is proportional to the amount of IFN-γ present, a stronger signal indicates a higher measured cytokine concentration within the assay comparison. This relationship converts molecular recognition into a quantitative readout rather than a simple presence-or-absence result.
IFN-γ is informative because activated immune cells release this cytokine. Measuring it after exposure to relevant antigens can indicate how strongly cellular immune responses are engaged under a given condition. In immunology and infection studies, the resulting concentration helps characterize immune activation and provides a quantitative marker for comparing responses.
A measurement begins by bringing the biological sample into contact with the antibody system, allowing IFN-γ to be captured and recognized by the enzyme-linked antibody. Substrate is then added to generate the color signal, which is interpreted as a cytokine concentration. This sequence links sample analysis, molecular detection, and quantitative comparison in one workflow.
The assay supplies a quantitative basis for comparing samples exposed to different experimental conditions. Their IFN-γ concentrations can be evaluated through the color-based readout, with differing values indicating changes in cellular immune response or activation. This approach is useful for examining antigen-related responses and monitoring differences across an experiment.
In infection research, the assay can help evaluate biological samples for responses associated with pathogen exposure, characterize inflammatory processes, and quantify immune activation. Its quantitative format also supports assessment of candidate vaccines or immunotherapies, where investigators need to compare cellular immune responses across experimental conditions and identify changes in cytokine production.