Ivis Technology
Ivis Technology - Related Videos
10:21
In Vivo Imaging Systems (IVIS) Detection of a Neuro-Invasive Encephalitic Virus
0 Views •
Cited by 11 •
2012
Utilizing luciferase and in vivo imaging systems (IVIS) as a novel means to identify disease endpoints before clinical developments occur. IVIS has allowed us to visualize in real time the invasion of encephalitic viruses over multiple days, providing a more accurate disease model for future study. It has also allowed us to identify the potential protective features of antivirals and vaccines faster than currently utilized animal models. The capability to utilize individual animals over...
08:53
In vivo Bioluminescent Imaging of Mammary Tumors Using IVIS Spectrum
0 Views •
Cited by 58 •
2009
Mammary tumor cells expressing luciferase are implanted subcutaneously in mice and visualized using optical imaging to monitor tumor growth and development non-invasively in a longitudinal study.
01:26
Technological Change
0 Views •
2024
Total Factor Productivity (TFP) measures the efficiency with which inputs are transformed into outputs in production. It is the essence of economic growth, driven by technological advancement. Consider the agricultural sector, where production requires vast amounts of human labor and work animals. Today, modern farm machinery and agriculture technologies have revolutionized how we cultivate crops and produce food much more efficiently. TP = A*f(K, L) TFP is represented as a multiplier 'A' in...
01:31
Hybridoma Technology
0 Views •
2023
Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy. Hybridoma Selection Commonly used fusion techniques — electroporation, polyethylene glycol...
07:06
Principles of Site-Specific Recombinase (SSR) Technology
0 Views •
Cited by 14 •
2008
The advent of site-specific recombinase (SSR) technology and the Cre/lox system has led to numerous advances in molecular biology, and has proven itself as a valuable tool for assessing gene function in transgenic animals. This interview discusses the mechanism of site specific recombination by Cyclization recombinase (Cre) and how the use of this enzyme has led to the development of conditional mutagenesis, which has significant advantages over traditional knock out strategies.
FAQs
IVIS technology detects light from either bioluminescent or fluorescent reporters, then uses a sensitive cooled charge-coupled device camera to capture and map signal intensity. The reporter type determines the light source being monitored, while the imaging system provides a spatial record of where signal occurs in the living subject. This supports visualization of biological activity without tissue collection.
Signal intensity provides a measurable indication of where reporter-associated activity is located within an animal model. Mapping that intensity helps researchers follow pathogen distribution, disease progression, or immune-cell-associated activity across different observations. Because the same subject can be assessed repeatedly, changes in signal can be related to progression or response over time rather than relying only on a single endpoint.
Luciferase-labeled microbes can help reveal where pathogens are distributed and how infection progresses in an animal model. Luciferase-labeled immune cells can similarly support visualization of changes in immune activity. Comparing these signals over time gives immunology and infection researchers a way to examine host responses alongside infection-related changes in the same living subject.
A typical workflow begins by using luciferase-labeled microbes or immune cells in an animal model. The living subject is then placed before the imaging system, where the cooled charge-coupled device camera captures emitted light and maps its intensity. Repeating imaging sessions over time produces longitudinal observations of pathogen distribution, disease progression, or immune activity.
Researchers can use IVIS technology when they need to monitor infection-related changes repeatedly during a study. Imaging may track pathogen distribution and disease progression while treatment is being evaluated, allowing signal patterns from the same subject to be compared across time. This provides a noninvasive way to assess infection control and treatment efficacy without requiring tissue collection at every observation.
Repeated imaging allows researchers to obtain multiple observations from the same living animal instead of relying exclusively on endpoint tissue collection. In immunology and infection studies, this can support monitoring of microbes, immune cells, and host responses across disease progression or treatment. The approach therefore helps reduce endpoint sampling and animal use while preserving longitudinal information about study outcomes.