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In vivo Bioluminescent Imaging of Mammary Tumors Using IVIS Spectrum
JoVE Journal
Biology
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JoVE Journal Biology
In vivo Bioluminescent Imaging of Mammary Tumors Using IVIS Spectrum

In vivo Bioluminescent Imaging of Mammary Tumors Using IVIS Spectrum

DOI:
10.3791/1210-v

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08:53 min

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April 29, 2009

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, ,
1Biology Research and Development ,Caliper Life Sciences

Chapters

  • 00:00Title
  • 00:48Introduction
  • 01:15Cell Preparation
  • 02:57Animal Injection
  • 04:06Animal Imaging
  • 06:27Time Course
  • 07:51Conclusion

Summary

Automatic Translation

Automatic Translation

Mammary tumor cells expressing luciferase are implanted subcutaneously in mice and visualized using optical imaging to monitor tumor growth and development non-invasively in a longitudinal study.

Tags

In Vivo Bioluminescent ImagingMammary TumorsIVIS Spectrum4T1 Mouse Mammary Tumor CellsXenograft Tumor ModelPre-clinical EvaluationAntitumor CompoundsFirefly Luciferase GeneLuciferin InjectionOptical Imaging SystemPhoton FluxAbsolute QuantitationLongitudinal StudiesTumor Growth MonitoringEarly Events In Tumor DevelopmentBioluminescent Signal ReductionTumor Cell DeathHypoxiaDrug Treatment

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